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Abstract : The present study was planned to investigate the effect of Desmodium gangeticum (L.) (DG) on lysosomal hydrolases, phosphatases and electrolytes in mechanically induced myocardial ischemic injury in rats. The isolated hearts of rats were subjected to global no-flow ischemia, followed by reperfusion in a Langendorff isolated perfused heart model. The group first hearts (n = 6) served as control where as group second hearts (n = 6) were subjected 30 min of global no-flow ischemia followed by 20 min of reperfusion. The group third and fourth hearts (n = 12) were subjected for preconditioning with DG, at the doses of 50, 100 mg kg -1, before 30 min starting the coronary occlusion followed by 20 min reperfusion. At the end of the study, heart were excised and lysosomal hydrolases, membrane bound phosphatases activities and electrolytes levels were assessed in the cardiac tissues. The activities of different lysosomal enzymes and hydrolases were increased (plt;0.05) significantly in the leakage as well as in heart tissue after ischemia-reperfusion injury. Whereas, in preconditioned heart with DG (50, 100 mg kg -1) significantly decreased activities of lysosomal hydrolases in the leakage as well as in the cardiac tissues were observed (plt;0.05). Similarly, significant (plt;0.05) decreased activity of membrane bound phosphatases such as Na7K + ATPase, Ca 2+ and Mg 2+ ATPase were also observed in the ischemic-reperfusion mediated injured hearts. It can be concluded that alteration in these enzyme activities may lead change in the electrolytes such as sodium, potassium and calcium content in the heart during ischemic reperfusion injury. However, DG preconditioned efficiently reversed the membrane-bound enzymes activity as well as maintained the normal electrolyte concentration. © 2012 Asian Network for Scientific Information.