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Fe O nanoparticles mediated synthesis of novel spirooxindole-dihydropyrimidinone molecules as Hsp90 inhibitors.

Publication Type : Journal Article

Publisher : Arch Pharm (Weinheim)

Source : Arch Pharm (Weinheim), Volume 352, Issue 1, p.e1800174 (2019)

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Keywords : Antineoplastic Agents, Antioxidants, cell proliferation, Dose-Response Relationship, Drug, Drug Screening Assays, Antitumor, Ferric Compounds, HEK293 Cells, Hep G2 Cells, HSP90 Heat-Shock Proteins, Humans, Magnetite Nanoparticles, MCF-7 Cells, Molecular Docking Simulation, Molecular Structure, Oxindoles, particle size, Pyrimidinones, Spiro Compounds, Structure-Activity Relationship, Surface properties

Campus : Kochi

School : School of Pharmacy

Department : Pharmaceutical Chemistry & Analysis

Year : 2019

Abstract : Heat shock protein 90 (Hsp90) is a validated molecular chaperone considered as the new key recipient for cancer intervention. The current study illustrates the synthesis of novel spirooxindole-dihydropyrimidinones (4a-j) by Fe O nanoparticles intervened synthesis and their Hsp90 ATPase inhibitory activity was investigated by the malachite green assay. All the compounds in the study demonstrated a moderate to potent ATPase inhibitory profile, with IC values ranging from 0.18 to 6.80 μM. Compounds 4j, 4h, 4f, and 4i exhibited maximum inhibitory potential with IC values of 0.18, 0.20, 0.35, and 0.55 μM, respectively. They were found to be better than the standard drug, geldanamycin (Hsp9 ATPase inhibition IC  = 0.90 μM). Compounds 4h and 4j with IC values of 22.82 ± 0.532, 20.78 ± 0.234 and 21.32 ± 0.765, 28.43 ± 0.653 µM showed significantly greater potencies against the MCF-7 and HepG2 cell lines, respectively. Compound 4j showed good antioxidant activities in the DPPH test and H O assay (IC  = 20.13.23 ± 0.32 and 23.27 ± 0.32 μg/mL) when compared with the standard ascorbic acid (IC  = 19.16 ± 0.20 and 20.66 ± 1.09 μg/mL). A molecular docking study was performed to observe the binding efficiency and steric interactions of the lead moiety.

Cite this Research Publication : S. Maddela, Makula, A., Galigniana, M. D., Parambi, D. G. T., Federicci, F., Mazaira, G., Hendawy, O. M., Dev, S., Mathew, G. E., and Bijo Mathew, “Fe O nanoparticles mediated synthesis of novel spirooxindole-dihydropyrimidinone molecules as Hsp90 inhibitors.”, Arch Pharm (Weinheim), vol. 352, no. 1, p. e1800174, 2019.

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