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Gene expression profiling of tuberculous meningitis

Publication Type : Journal Article

Publisher : Journal of Proteomics and Bioinformatics

Source : Journal of Proteomics and Bioinformatics, Volume 4, Number 5, p.98-105 (2011)

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Keywords : article, autopsy, beta1 integrin, brain tissue, cathepsin L, cathepsin l1, cathepsin L1 gene, CD134 antigen, chitinase 3 like 1, chitinase 3 like 1 gene, clinical article, controlled study, CXCL9 chemokine, CXCL9 gene, defensin alpha 3 neutrophil specific protein, defensin alpha 3 neutrophil specific protein gene, DNA microarray, down regulation, endosome, gene, Gene expression profiling, gene function, gene identification, gene product, genome analysis, glial fibrillary acidic protein, glial fibrillary acidic protein gene, HLA DR antigen, HLA DRB1 gene, human, human genome, human tissue, immunohistochemistry, integrin beta 1 gene, interleukin 12 receptor beta1, interleukin 12 receptor beta1 gene, interleukin 4 induced 1, interleukin 4 induced 1 gene, metallothionein I, metallothionein IF, metallothionein IF gene, natural resistance associated macrophage protein 1, nucleotide sequence, peptide transporter 2, protein localization, proto oncogene, proto oncogene MET, proto oncogene protein MET, serine peptidase inhibitor clade A member 3, serine peptidase inhibitor clade A member 3 gene, serine proteinase inhibitor, SERPINA3, SLC11A1 gene, SLC15A2 gene, sorting nexin, sorting nexin 12, sorting nexin 12 gene, sorting nexin 18, sorting nexin 18 gene, sorting nexin 3, sorting nexin 3 gene, Transcriptome, tuberculous meningitis, tumor necrosis factor receptor superfamily member 4 gene, unclassified drug, upregulation

Campus : Bengaluru

School : School of Biotechnology

Department : Bioinformatics

Year : 2011

Abstract : Tuberculous meningitis (TBM) is a form of extra pulmonary tuberculosis that is associated with severe neurological deficits and a high mortality. Early diagnosis of TBM is a major challenge despite the availability of several diagnostic methods. Existing diagnostic methods and markers are inadequate for early diagnosis of TBM owing to poor specificity and sensitivity. DNA microarray technology permits high-throughput identification of differentially expressed genes. In order to identify molecules as candidate biomarkers for early diagnosis or as therapeutic targets in TBM, we carried out transcriptomic analysis of brain tissue using whole human genome oligonucleotide arrays. From this gene expression analysis, we identified 2,434 genes that were differentially expressed at least two-fold in TBM cases as compared to controls. The large majority of the differentially expressed genes encoded proteins that are involved in metabolism, cell growth, transport, immune response, cell communication and signal transduction. We confirmed the upregulation of two molecules, serpin peptidase inhibitor, clade A member 3 (SERPINA3) and glial fibrillary acidic protein (GFAP), at the protein level by immunohistochemical analysis. The findings from our study should help us understand the molecular mechanisms underlying TBM and to develop better diagnostic and therapeutic strategies against this deadly disease. © 2011 Kumar GSS, et al.

Cite this Research Publication : G. Sab Sameer Kumar, Venugopal, A. Kab c d, Selvan, L. D. Nae, A. Marimuthu, Keerthikumar, Sa, Pathare, Sg, Dikshit, J. Bg, Tata, Pg, Hariharan, Rg, Prasad, T. S. Ka, H. C. Harsha, Ramachandra, Y. Lb, Mahadevan, Ah, Chaerkady, Rab c d, Shankar, S. Kh, and Pandey, Acd i j, “Gene expression profiling of tuberculous meningitis”, Journal of Proteomics and Bioinformatics, vol. 4, pp. 98-105, 2011.

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