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Immunochemical characterisation of Mycobacterium Tuberculosis H-37 Ra antigen

Publication Type : Journal Article

Thematic Areas : Medical Sciences

Publisher : Indian Medical Gazette

Source : Indian Medical Gazette , 1996.

Campus : Kochi

School : School of Medicine

Year : 1996

Abstract : The saline extract (SE) of Mycobacterium tuberculosis H37Ra yielded four major fractions on Sephadex G-100, with average molecular weights of 48,000 (fraction I, F I) 32,000 (fraction II, F II), 15,000 (fraction III, F III) and 5,000 (fraction IV, F IV). FI and FII gave single bands on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, showed complete immunological cross-reactivity with each other and were more potent in reacting with antibodies in patients' (tuberculosis) sera than sonicate, SE, purified protein derivative, F III and F IV when tested in a competition enzyme-linked immunosorbent assay.

Cite this Research Publication : S. Vivekanandhan, Chawla, T. C., Dr. Damodaran Vasudevan, and Maheswari, M. C., “Immunochemical characterisation of Mycobacterium Tuberculosis H-37 Ra antigen”, Indian Medical Gazette , 1996.

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