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Immunoelectrophoresis of mycobacterial antigens

Publication Type : Journal Article

Source : Indian Journal Exp. Biol 1990

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Campus : Kochi

School : School of Medicine

Year : 1990

Abstract : Polyvalent antiserum to culture filtrate of H37 Ra M. tuberculosis was raised in rabbits. Monospecific antiserum was raised against M. tuberculosis antigen-5, prepared from the culture filtrates by immunoabsorbent affinity chromatography. On immunoelectrophoresis, antigen-5 demonstrated single precipitin arc against polyvalent and monospecific antisera. The culture filtrate antigen demonstrated multiple precipitin arcs against polyvalent antiserum and single precipitin are against monospecific antiserum. Antigen-5 could be isolated and characterized from the culture filtrate of H37 Ra M. tuberculosis. Immunoelectrophoresis could be one of the method to characterize the mycobacterial antigens prepared in the laboratory.

Cite this Research Publication : Radhakrishnan V.V, Annamma M, Rao SB, Sehgal S. Immunoelectrophoresis of mycobacterial antigens. Indian J Exp. Biol 1990;28:812-815.

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