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Injectable Angiogenic and Osteogenic Carrageenan Nanocomposite Hydrogel for Bone Tissue Engineering

Publication Type : Journal Article

Thematic Areas : Nanosciences and Molecular Medicine

Publisher : International Journal of Biological Macromolecules

Source : International Journal of Biological Macromolecules, Volume 122, p.320 - 328 (2019)

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Keywords : angiogenesis, Carrageenan hydrogel, Dimethyloxallylglycine, Osteogenesis, Whitlockite nanoparticles

Campus : Kochi

School : Center for Nanosciences

Center : Amrita Center for Nanosciences and Molecular Medicine Move, Nanosciences

Department : Nanosciences and Molecular Medicine

Year : 2019

Abstract : Functional biomaterials that couple angiogenesis and osteogenesis processes are vital for bone tissue engineering and bone remodeling. Herein we developed an injectable carrageenan nanocomposite hydrogel incorporated with whitlockite nanoparticles and an angiogenic drug, dimethyloxallylglycine. Synthesized whitlockite nanoparticles and nanocomposite hydrogels were characterized using SEM, TEM, EDS and FTIR. Developed hydrogels were injectable, mechanically stable, cytocompatible and has better protein adsorption. Incorporation of dimethyloxallylglycine resulted in initial burst release followed by sustained release for 7 days. Human umbilical vein endothelial cells exposed to dimethyloxallylglycine incorporated nanocomposite hydrogel showed enhanced cell migration and capillary tube-like structure formation. Osteogenic differentiation in rat adipose derived mesenchymal stem cells after 7 and 14 days revealed increased levels of alkaline phosphatase activity in vitro. Furthermore, cells exposed to nanocomposite hydrogel revealed enhanced protein expressions of RUNX2, COL and OPN. Overall, these results suggest that incorporation of whitlockite and dimethyloxallylglycine in carrageenan hydrogel promoted osteogenesis and angiogenesis in vitro.

Cite this Research Publication : R. Yegappan, Selvaprithiviraj, V., Sivashanmugam Amirthalingam, Mohandas, A., Nathaniel S. Hwang, and Dr. Jayakumar Rangasamy, “Injectable Angiogenic and Osteogenic Carrageenan Nanocomposite Hydrogel for Bone Tissue Engineering”, International Journal of Biological Macromolecules, vol. 122, pp. 320 - 328, 2019.

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