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Layered Chitosan-collagen Hydrogel/aligned PLLA Nanofiber Construct for Flexor Tendon Regeneration.

Publication Type : Journal Article

Thematic Areas : Nanosciences and Molecular Medicine

Publisher : Carbohydr Polym.

Source : Carbohydr Polym, Volume 153, p.492-500 (2016)

Keywords : Animals, Biocompatible Materials, cell proliferation, Cells, Cultured, chitosan, collagen, Hydrogel, Polyethylene Glycol Dimethac.rylate, Nanofibers, Polyesters, Rabbits, regeneration, Tendons, Tensile strength, tissue engineering, Tissue Scaffolds

Campus : Kochi

School : Center for Nanosciences

Center : Amrita Center for Nanosciences and Molecular Medicine Move, Nanosciences

Department : Nanosciences and Molecular Medicine

Year : 2016

Abstract : The aim of our study was to develop a tendon construct of electrospun aligned poly (l-lactic acid) (PLLA) nanofibers, to mimic the aligned collagen fiber bundles and layering PLLA fibers with chitosan-collagen hydrogel, to mimic the glycosaminoglycans of sheath ECM for tendon regeneration. The hydrogel coated electrospun membrane was rolled and an outer coating of alginate gel was given to prevent peritendinous adhesion. The developed constructs were characterized by SEM, FT-IR and tensile testing. Protein adsorption studies showed lower protein adsorption on coated scaffolds compared to uncoated scaffolds. The samples were proven to be non-toxic to tenocytes. The chitosan-collagen/PLLA uncoated scaffolds and alginate gel coated chitosan-collagen/PLLA scaffolds showed good cell proliferation. The tenocytes showed good attachment and spreading on the scaffolds. This study indicated that the developed chitosan-collagen/PLLA/alginate scaffold would be suitable for flexor tendon regeneration.

Cite this Research Publication : S. Deepthi, M Sundaram, N., J Kadavan, D., and Jayakumar, R., “Layered chitosan-collagen hydrogel/aligned PLLA nanofiber construct for flexor tendon regeneration.”, Carbohydr Polym, vol. 153, pp. 492-500, 2016.

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