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Magnetic Resonance Functional Nano-hydroxyapatite Incorporated Poly(caprolactone) Composite Scaffolds for in Situ Monitoring of Bone Tissue Regeneration by MRI

Publication Type : Journal Article

Thematic Areas : Medical Sciences, Nanosciences and Molecular Medicine

Publisher : Tissue Engineering - Part A

Source : Tissue Engineering - Part A, Mary Ann Liebert Inc., Volume 20, Number 19-20, p.2783-2794 (2014)

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Keywords : adsorption, article, biological activity, Bone, bone development, Bone regeneration, Bone tissue engineering, Bone tissue regeneration, Cell culture, cell differentiation, cell proliferation, Contact angle, contrast enhancement, controlled study, Electrospinning, gadolinium, human, human cell, Human mesenchymal stem cells (hMSCs), Hydroxyapatite, Hydroxyapatite nanocrystals, Hydroxyapatite nanoparticles, Imaging techniques, in vitro study, leaching, Magnetic resonance, Mechanics, mesenchymal stem cell, molecular imaging, molecular scaffold, multifunctional hydroxyapatite nanoparticle, Nanocomposites, Nanofibers, Nanofibrous scaffolds, nuclear magnetic resonance imaging, nuclear magnetic resonance scanner, Osteogenic differentiation, particle size, physical chemistry, polycaprolactone, protein, Scaffolds (biology), Stem cells, strength, Tissue, Tissue engineered constructs, Tissue regeneration, unclassified drug

Campus : Kochi

School : Center for Nanosciences, School of Medicine

Center : Amrita Center for Nanosciences and Molecular Medicine Move, Nanosciences

Department : Nanosciences, Radiodiagnosis

Year : 2014

Abstract : In this study, we have reported the incorporation of a multi-modal contrast agent based on hydroxyapatite nanocrystals, within a poly(caprolactone)(PCL) nanofibrous scaffold by electrospinning. The multifunctional hydroxyapatite nanoparticles (MF-nHAp) showed simultaneous contrast enhancement for three major molecular imaging techniques. In this article, the magnetic resonance (MR) contrast enhancement ability of the MF-nHAp was exploited for the purpose of potentially monitoring as well as for influencing tissue regeneration. These MF-nHAp containing PCL scaffolds were engineered in order to enhance the osteogenic potential as well as its MR functionality for their application in bone tissue engineering. The nano-composite scaffolds along with pristine PCL were evaluated physico-chemically and biologically in vitro, in the presence of human mesenchymal stem cells (hMSCs). The incorporation of 30-40 nm sized MF-nHAp within the nanofibers showed a substantial increase in scaffold strength, protein adsorption, proliferation, and osteogenic differentiation of hMSCs along with enhanced MR functionality. This preliminary study was performed to eventually exploit the MR contrast imaging capability of MF-nHAp in nanofibrous scaffolds for real-time imaging of the changes in the tissue engineered construct. © Copyright 2014, Mary Ann Liebert, Inc.

Cite this Research Publication : N. Ganesh, Anusha Ashokan, R kannan, R., Chennazhi, K., Shantikumar V Nair, and Dr. Manzoor K., “Magnetic Resonance Functional Nano-hydroxyapatite Incorporated Poly(caprolactone) Composite Scaffolds for in Situ Monitoring of Bone Tissue Regeneration by MRI”, Tissue Engineering - Part A, vol. 20, pp. 2783-2794, 2014.

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