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Nano Polydopamine Crosslinked thiol-functionalized Hyaluronic Acid Hydrogel for Angiogenic Drug Delivery

Publication Type : Journal Article

Thematic Areas : Nanosciences and Molecular Medicine

Publisher : Colloids Surf B Biointerfaces

Source : Colloids Surf B Biointerfaces, Volume 177, p.41-49 (2019)

Campus : Kochi

School : Center for Nanosciences

Center : Amrita Center for Nanosciences and Molecular Medicine Move, Nanosciences

Department : Nanosciences and Molecular Medicine

Year : 2019

Abstract : Crosslinking of polymeric network using nanoparticles by physical or chemical method to obtain hydrogel is an emerging approach. Herein, we synthesized Polydopamine (PDA) nanoparticles via oxidative self-polymerization of dopamine in water-ethanol mixture. Thiol-functionalized hyaluronic acid was developed using cysteamine and hyaluronic acid (HA-Cys) via 1-Ethyl-3-(3-Dimethylaminopropyl) Carbodiimide - N-hydroxysuccinimide (EDC-NHS) crosslinking chemistry. Developed HA-Cys conjugate was cross-linked using PDA nanoparticles via Michael-type addition reaction. Synthesized nanoparticles were monodisperse with size of 124 ± 8 nm and had spherical morphology. FTIR characterization confirmed successful synthesis of HA-Cys conjugate and subsequent crosslinking with PDA nanoparticles. Rheological characterization revealed that hydrogels were injectable in nature with good mechanical stability. Dimethyloxalylglycine (DMOG) loaded PDA nanoparticle showed sustained drug release for period of 7 days from composite hydrogel. Hydrogel microenvironment facilitated enhanced endothelial cell migration, proliferation and attachment. Furthermore, in response to release of DMOG from developed hydrogel, cells showed enhanced capillary tube formation in vitro. Overall, these results demonstrate that PDA cross-linked thiol-functionalized hydrogel was developed in a facile manner under physiological conditions. These developed hydrogels could be potentially used in tissue engineering and drug delivery.

Cite this Research Publication : R. Yegappan, Selvaprithiviraj, V., Mohandas, A., and Dr. Jayakumar Rangasamy, “Nano Polydopamine Crosslinked thiol-functionalized Hyaluronic Acid Hydrogel for Angiogenic Drug Delivery”, Colloids Surf B Biointerfaces, vol. 177, pp. 41-49, 2019.

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