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Paper-based Device for the Colorimetric Assay of Bilirubin Based on in-situ Formation of Gold Nanoparticles

Publication Type : Journal Article

Thematic Areas : Biotech

Publisher : Microchimica Acta

Source : Microchimica Acta, vol. 187, p. 60, 2019

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Keywords : Conjugated bilirubin, gold nanoparticle, In situ reduction, Machine learning classifiers, Optical detection, Paper-based colorimetric assay, Random Forest classificationRGB and CYMK color model, surface plasmon resonance, Wax printing

Campus : Amritapuri, Coimbatore

School : School of Biotechnology, School of Engineering

Center : Biosensor Research Lab, Biotechnology

Department : biotechnology

Year : 2019

Abstract : A paper-based colorimetric assay for the determination of bilirubin has been developed. The method is based on the in-situ reduction of chloroauric acid to form gold nanoparticles. A chromatographic paper was patterned using a wax printer. Chloroauric acid was drop-cast onto the reagent zone. In the presence of bilirubin, gold(III) ions are reduced and form gold nanoparticles. This leads to a color change from yellow to purple. The intensity of the purple color (peak at 530 nm) increases with bilirubin concentration in the 5.0 to 1000 mg L−1 range. The detection limit is 1.0 mg L−1. For the quantification of bilirubin, images were captured using a digital camera, and data were processed with the help of machine learning-based supervised prediction using Random Forest classification. The method was applied to the determination of bilirubin in urine samples. The spiked urine samples exhibit more than 95% recovery.

Cite this Research Publication : R. P. Edachana, Kumaresan, A., Balasubramanian, V., Thiagarajan, R., Dr. Bipin G. Nair, and Dr. Satheesh Babu T. G., “Paper-based device for the colorimetric assay of bilirubin based on in-situ formation of gold nanoparticles”, Microchimica Acta, vol. 187, p. 60, 2019

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