To find out the pytochemical constituents in the seeds of Luffa cylindrica and determine their anti-inflammatory, Bronchodilator and Antimicrobial activity. The fruit of Luffa cylindrica collected from the village, Othakadai near Madurai, Tamilnadu, south India were dried and the seeds were separated from the fruit and extracted with petroleumether, benzene, chloroform and alcohol. The petroleum ether extract and benzene extract were mixed and chromatographed, by using solvents n-Hexane, petroleum ether, benzene, ethylacetate and methanol. Finally the compounds isolated Cu-1, Cu-2, Cu-3 and Cu-4. Phytochemical screening was carried out according to standard procedures. Anti inflammatory activity was determined by carragenan induced paw-odema method by using external standard Diclofenoc sodium and oral Brufen. Bronchodilator activity using Guinea pig trachea compared to standard Aminophylline. Anti microbial activity was effective against S. aureus and Candida albicans. For all the activities the column isolated compounds and alcohol, petroleum ether extracts were used. Sugar, protein, Alkaloids, flavonoids, sterols and glycoside were found to be present in the extracts. Cu-1 has moderate anti-inflammatory and Cu-3 has significant anti-inflammatory activity. Cu-2 and Cu-4 have significant antibacterial and antifungal activity. Cu-4 has significant Bronchodilator activity. Luffa cylindrica seed extracts and oil possess good anti inflammatory, Bronchodilator and antimicrobial activity.
P. Muthumani, Meera, R., Dr. Subin Mary Zachariah, ,, Devi, P., Kameswari, B., and Priya, B. E., “Phytochemical screening and anti inflammatory, bronchodilator and antimicrobial activities of the seeds of Luffa cylindrica.”, Research Journal of Pharmaceutical, Biological and Chemical Sciences, vol. 1, pp. 11-22, 2010.