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Preparation, Characterization, Bioactive and Cell Attachment Studies of α-chitin/gelatin Composite Membranes

Publication Type : Journal Article

Thematic Areas : Nanosciences and Molecular Medicine

Publisher : International Journal of Biological Macromolecules

Source : International Journal of Biological Macromolecules, Volume 44, Number 4, p.333-337 (2009)

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Keywords : alpha chitin, article, Artificial, biocompatibility, body fluid, Calcium phosphate, cell adhesion, Cell Line, cell membrane, Cells, chitin, chitin derivative, drug activity, Electron, enzyme degradation, gelatin, human, human cell, Humans, hydrogel, Hydrogels, incubation time, Membranes, microscopy, osteoblast, Scanning, scanning electron microscopy, simulation, swelling, tissue engineering, unclassified drug, X ray diffraction, X-Ray Diffraction

Campus : Kochi

School : Center for Nanosciences

Center : Amrita Center for Nanosciences and Molecular Medicine Move, Nanosciences

Department : Nanosciences and Molecular Medicine

Year : 2009

Abstract : The chitin/gelatin composite membranes were prepared by mixing of chitin hydrogel with gelatin. The prepared composite membranes were characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD), mechanical, swelling, enzymatic degradation and thermal studies. The XRD pattern of the chitin/gelatin composite membranes showed almost the same pattern as α-chitin. The bioactivity studies of these chitin/gelatin membranes were carried out with the simulated body fluid solution (SBF) for 7, 14 and 21 days followed by the characterization with the scanning electron microscopy (SEM) and Energy Dispersive Spectrum (EDS) studies. The SEM and EDS studies confirmed the formation of calcium phosphate layer on the surface of chitin/gelatin membranes. Biocompatibility of the chitin/gelatin membrane was assessed using human MG-63 osteoblast-like cells. After 48 h of incubation, it was found that the cells had attached and completely covered the membrane surface. Thus, the prepared chitin/gelatin membranes are bioactive and are suitable for cell adhesion suggesting that these membranes can be used for tissue-engineering applications.

Cite this Research Publication : H. Nagahama, Rani, V. V. D., Shalumon, K. T., Dr. Jayakumar Rangasamy, Nair, S. V., Koiwa, S., Furuike, T., and Tamura, H., “Preparation, Characterization, Bioactive and Cell Attachment Studies of α-chitin/gelatin Composite Membranes”, International Journal of Biological Macromolecules, vol. 44, pp. 333-337, 2009.

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