Publication Type : Journal Article
Thematic Areas : Nanosciences and Molecular Medicine
Publisher : Carbohydrate Polymers
Source : Carbohydrate Polymers, Volume 84, Number 1, p.407-416 (2011)
Url : http://www.scopus.com/inward/record.url?eid=2-s2.0-78951480365&partnerID=40&md5=04f8ddf46b89b3556465802da9d3f721
Keywords : AFM, Anti cancer saponin delivery, Cancer cell lines, Cell culture, Cell lines, chitin, chitosan, Chitosan nanoparticles, Concentration ranges, cytotoxicity, FTIR, In-vitro, Metabolites, Nanoparticles, Nanosaponin, Saponin, SEM, Specific toxicity, Sustained release, Therapeutic agents
Campus : Kochi
School : Center for Nanosciences
Center : Amrita Center for Nanosciences and Molecular Medicine Move, Nanosciences
Department : Nanosciences and Molecular Medicine
Year : 2011
Abstract : In this work we developed a nanoformulation for anticancer saponin with chitosan for an enhanced and sustained release. The saponin loaded chitosan nanoparticles showed a particle size of 65 ± 7 nm. The synthesized nanoparticles were analyzed by FTIR, TG/DTA, SEM and AFM. The cytotoxicity of the nanoparticles was analyzed on L929, NIH-3T3, KB and PC3 which showed particles are non-toxic in a concentration range of 0.1-1.0 mg/ml whereas the nanosaponin showed specific toxicity on PC3 and KB cell lines. The internalization of the nanosaponin on L929 and PC3 was confirmed by Rhodamine conjugation with the nanoparticles. Our preliminary studies support that nanosaponin could be an efficient therapeutic agent for cancer.
Cite this Research Publication : N. S. Rejinold, Muthunarayanan, M., Muthuchelian, K., Chennazhi, K. P., Nair, S. V., and Dr. Jayakumar Rangasamy, “Saponin-Loaded Chitosan Nanoparticles and their Cytotoxicity to Cancer Cell Lines in Vitro”, Carbohydrate Polymers, vol. 84, pp. 407-416, 2011.