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Self-calibrated fluorescent probe resembled as an indicator of the lysosomal phosphatase pertaining to the cancer cells.

Publication Type : Journal Article

Thematic Areas : Advanced Materials and Green Technologies

Publisher : Journal of Photochemistry and Photobiology B: Biology

Source : Journal of Photochemistry and Photobiology B: Biology, Volume 177, p.105-111 (2017)

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Keywords : Animals, calibration, Cell Survival, Fluorescent Dyes, HCT116 Cells, Humans, Lysosomes, Mice, Microscopy, Fluorescence, NIH 3T3 Cells, Phosphoric Monoester Hydrolases, Spectrometry, Fluorescence

Campus : Coimbatore

School : School of Engineering

Center : Center for Excellence in Advanced Materials and Green Technologies, Center for Industrial Research and Innovation (ACIRI)

Department : Civil

Year : 2017

Abstract : A self-calibrated fluorescent probe Lyso-Phos has synthesized followed by a straightforward synthetic pathway. Lyso-Phos acts as an indicator for lysosomal phosphatase. Its photophysical property including cellular imaging was described. Lyso-Phos showed ratiometric UV-Vis- absorption changes from λ 370nm to λ 450nm in the presence of alkaline phosphatase (ALP). On the other hand, fluorescence intensity λ 560nm of Lyso-Phos has increased around 45-fold in the presence of ALP. The probe Lyso-Phos was found to be highly chemoselective toward the phosphatase compared with other ubiquitous entities in cellular milieu. The non-toxic nature of the Lyso-phos has accounted by observing higher cell viability in prostate cancer- LnCap, fibrosarcoma HT1080 and normal mouse embryo fibroblast NIH3T3 cells. Further, the probe Lyso-Phos was utilized for tracking of cellular phosphatase in live-cells. Lyso-Phos enabled to track cellular phosphatase by the extent of fluorescence labeling of LnCap cells which showed reasonable uptake efficiency in the presence of Lyso-Phos as indicated by the intracellular fluorescence. The phosphoester bond in the probe was cleaved by intracellular alkaline phosphatase leading to turn on fluorescence of the fluorescent probe Lyso-Phos. Finally, cellular colocalization with Lyso-Tracker empowered our speculation that Lyso-Phos can track endogenous phosphatase in the lysosomes. Altogether these findings suggest that Lyso-Phos would be powerful probe to detect phosphates in cancer cells.

Cite this Research Publication : A. Podder, Alex, S. M., Maiti, M., Maiti, K. Kumar, and Bhuniya, S., “Self-calibrated fluorescent probe resembled as an indicator of the lysosomal phosphatase pertaining to the cancer cells.”, Journal of Photochemistry and Photobiology B: Biology, vol. 177, pp. 105-111, 2017.

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