Publication Type : Journal Article
Source : Journal Infect Dis Immun: 2012
Campus : Kochi
Year : 2012
Abstract : In this study, histopathological features of pulmonary granuloma induced by trehalose 6, 6’dimycolate
(TDM) of Mycobacterium tuberculosis bacilli in naive and lymphopenic mice are documented.
Simultaneously, (a) proinflammatory cytokine (tumour necrosis factor alpha -TNF-α) and macrophage
chemotactic factor (macrophage inflammatory protein 1 alpha – MIP-1α) concentrations in the lung
homogenates were estimated by a sandwich enzyme-linked immunosorbent assay (ELISA), (b)
chemotactic response of mouse peritoneal macrophages to TDM, are assayed. Non-caseating
histiocytic granulomas in lungs as well as elevated TNF-α and MIP-1α concentrations in the lung
homogenates were observed in naive mice. By contrast, lymphopenic mice showed no demonstrable
granulomatous response to TDM and there was marked decrease in TNF-α and MIP1-α concentrations
in the lung homogenates. The peritoneal macrophages of naive mice demonstrated optimum
chemotactic response to TDM in vitro, while peritoneal macrophages of lymphopenic mice showed
suboptimal chemotactic response to TDM. The results of this experimental study indicated that: (a) the
pulmonary granuloma in mouse following TDM administration depends not only upon the production of
TNF-α and MIP-1α in optimal amounts by the host, but also their participation during the active stages
of granuloma formation; (b) TDM promotes chemotaxis of peritoneal macrophages in naive mice in vitro
and this is facilitated by the MIP-1α
Cite this Research Publication : Annamma M, Neelima R, Harikrishnan V.S, Radhakrishnan VV. Trehalose 6,6’ dimycolate (cord factor) induced pulmonary granuloma in naïve and lymphopenic mice. J Infect Dis Immun: 2012; 4 (3):29-35.