'Advanced Instrumentation Techniques' is a course offered in the eighth semester of B. Pharm program at School of Pharmacy, Health Sciences campus, Amrita Vishwa Vidyapeetham.
Course Duration: 45 Hours
This subject deals with the application of instrumental methods in qualitative and quantitative analysis of drugs. This subject is designed to impart advanced knowledge on the principles and instrumentation of spectroscopic and chromatographic hyphenated techniques. This also emphasiz- es on theoretical and practical knowledge on modern analytical instruments that are used for drug testing.
Upon completion of the course the student shall be able to
Unit I (10 Hours)
Nuclear Magnetic Resonance spectroscopy: Principles of H-NMR and C-NMR, chemical shift, factors affecting chemical shift, coupling constant, Spin - spin coupling, relaxation, instrumentation and applications
Mass Spectrometry- Principles, Fragmentation, Ionization techniques – Electron impact, chemical ioniza- tion, MALDI, FAB, Analyzers-Time of flight and Quadrupole, instrumentation, applications
Unit II (10 Hours)
Thermal Methods of Analysis: Principles, instrumentation and applications of ThermogravimetricAnaly- sis (TGA), Differential Thermal Analysis (DTA), Differential Scanning Calorimetry (DSC)
X-Ray Diffraction Methods: Origin of X-rays, basic aspects of crystals, Xray Crystallography, rotating crystal technique, single crystal diffraction,powder diffraction, structural elucidation and applications.
Unit III (10 Hours)
Calibration and validation-as per ICH and USFDA guidelines Calibration of following Instruments Electronic balance, UV-Visible spectrophotometer, IR spectrophotometer, Fluorimeter, Flame Photometer, HPLC and GC
Unit IV (08 Hours)
Radio immune assay: Importance, various components, Principle, different methods, Limitation and Applications of Radio immunoassay
Extraction techniques: General principle and procedure involved in the solid phase extraction and liquid liquid extraction
Unit V (07 Hours)
Hyphenated techniques-LC-MS/MS, GC-MS/MS, HPTLC-MS.
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