Unit I (10 Hours)
Nuclear Magnetic Resonance spectroscopy: Principles of H-NMR and C-NMR, chemical shift, factors affecting chemical shift, coupling constant, Spin - spin coupling, relaxation, instrumentation and applications
Mass Spectrometry- Principles, Fragmentation, Ionization techniques – Electron impact, chemical ioniza- tion, MALDI, FAB, Analyzers-Time of flight and Quadrupole, instrumentation, applications
Unit II (10 Hours)
Thermal Methods of Analysis: Principles, instrumentation and applications of ThermogravimetricAnaly- sis (TGA), Differential Thermal Analysis (DTA), Differential Scanning Calorimetry (DSC)
X-Ray Diffraction Methods: Origin of X-rays, basic aspects of crystals, Xray Crystallography, rotating crystal technique, single crystal diffraction,powder diffraction, structural elucidation and applications.
Unit III (10 Hours)
Calibration and validation-as per ICH and USFDA guidelines Calibration of following Instruments Electronic balance, UV-Visible spectrophotometer, IR spectrophotometer, Fluorimeter, Flame Photometer, HPLC and GC
Unit IV (08 Hours)
Radio immune assay: Importance, various components, Principle, different methods, Limitation and Applications of Radio immunoassay
Extraction techniques: General principle and procedure involved in the solid phase extraction and liquid liquid extraction
Unit V (07 Hours)
Hyphenated techniques-LC-MS/MS, GC-MS/MS, HPTLC-MS.