| Course Name | Flow Cytometry Cell sorting and Applications |
| Course Code | 25CMD511 |
| Program | M. Sc. in Advanced Clinical and Molecular Diagnostics |
| Semester | 2 |
| Credits | 3 |
| Campus | Faridabad |
Unit 1:
Introduction to Flow Cytometry, Overview of flow cytometry and its historical development, Basic components of a flow cytometer: fluidics, optics, and electronics, Principles of fluorescence and fluorochrome selection.
Unit 2:
Instrumentation and Operation, Detailed study of flow cytometer components and their functions, Calibration, compensation, and quality control procedures, Hands-on training in instrument startup, operation, and shutdown protocols.
Unit 3:
Experimental Design and Multicolor Panel Development, Strategies for designing flow cytometry experiments, Selection of antibodies and fluorochromes for multicolor panels, Controls and standards in flow cytometry assays.
Unit 4:
Data Acquisition and Analysis, Techniques for data acquisition and gating strategies, Use of flow cytometry software for data analysis, Interpretation of histograms, dot plots, and statistical outputs.
Unit 5:
Cell Sorting Techniques, Principles and mechanics of fluorescence-activated cell sorting (FACS), Sorting strategies: purity vs. yield considerations, Practical sessions on setting up and performing cell sorting experiments.
Unit 6:
Applications of Flow Cytometry, Immunophenotyping and analysis of immune cell subsets, Assessment of cell cycle, apoptosis, and proliferation, Applications in stem cell research and cancer immunology.
(45 classes)
Preamble
Flow Cytometry Cell Sorting and Applications is a graduate-level course offering a comprehensive overview of flow cytometry technologies and their use in research and clinical settings. The course explores principles of fluorescence, antibody labeling, multi-parametric analysis, and cell sorting techniques. Applications discussed include immunophenotyping, cell cycle analysis, apoptosis detection, and stem cell characterization.
Course Outcome:
CO1: Understand the principles and components of flow cytometry.
CO2: Operate flow cytometers, including calibration and quality control.
CO3: Design flow cytometry experiments with multicolor panels.
CO4: Acquire and analyze data using gating strategies and flow cytometry software.
CO5: Master FACS techniques for sorting cells based on purity and yield.
CO6: Apply flow cytometry in immunophenotyping, cell cycle analysis, apoptosis, and stem cell research.
Program outcome
PO1: Bioscience Knowledge
PO2: Problem Analysis
PO3: Design/Development of Solutions
PO4: Conduct Investigations of complex problems
PO5: Modern tools usage
PO6: Bioscientist and Society
PO7: Environment and Sustainability
PO8: Ethics
PO9: Individual & Team work
PO10: Communication
PO11: Project management & Finance
PO12: Lifelong learning
0 – No affinity; 1 – low affinity; 2 – Medium affinity; 3 – High affinity
| Course Outcome (CO) | PO1 | PO2 | PO3 | PO4 | PO5 | PO6 | PO7 | PO8 | PO9 | PO10 | PO11 | PO12 |
| CO1 | 3 | 2 | 2 | 2 | 3 | 1 | 0 | 1 | 1 | 2 | 1 | 2 |
| CO2 | 3 | 2 | 2 | 3 | 3 | 1 | 0 | 1 | 1 | 2 | 2 | 2 |
| CO3 | 3 | 3 | 3 | 3 | 3 | 1 | 0 | 1 | 1 | 3 | 1 | 2 |
| CO4 | 3 | 3 | 2 | 3 | 3 | 1 | 0 | 1 | 2 | 3 | 1 | 3 |
| CO5 | 3 | 3 | 3 | 3 | 3 | 2 | 0 | 1 | 2 | 2 | 1 | 3 |
| CO6 | 3 | 3 | 3 | 3 | 3 | 2 | 0 | 1 | 3 | 3 | 1 | 3 |
Program-specific outcome
PSO 1 – Emerging technologies in clinical diagnostics
PSO 2 – Biomolecules in Medicine
PSO 3 – Molecular dysregulation in diseases
PSO 4 – Molecular technology in diagnosis and therapy
PSO 5 – Applying lab discoveries to clinical practice
PSO 6 – Microorganisms in Medicine
PSO 7 – Statistical methods to interpret and validate diagnostic results
PSO 8 – Integrate molecular diagnostics into personalized medicine
PSO 9 – Compounds as biomarkers and its specificity
PSO 10 – Bioinformatics and biological data use
0 – No affinity; 1 – low affinity; 2 – Medium affinity; 3 – High affinity
| CO/PSO | PSO 1 | PSO 2 | PSO 3 | PSO 4 | PSO 5 | PSO 6 | PSO 7 | PSO 8 | PSO 9 | PSO 10 |
| CO1 | 3 | 2 | 1 | 3 | 3 | 1 | 1 | 3 | 2 | 2 |
| CO2 | 3 | 2 | 1 | 3 | 3 | 1 | 1 | 3 | 2 | 2 |
| CO3 | 3 | 3 | 2 | 3 | 3 | 1 | 1 | 3 | 2 | 3 |
| CO4 | 3 | 2 | 1 | 3 | 3 | 1 | 2 | 3 | 2 | 3 |
| CO5 | 3 | 3 | 2 | 3 | 3 | 2 | 1 | 3 | 2 | 3 |
| CO6 | 3 | 3 | 2 | 3 | 3 | 2 | 1 | 3 | 3 | 3 |
Textbook:
Ormerod MG. Flow Cytometry: A Practical Approach. 3rd ed. Oxford: Oxford University Press; 2000.
Reference Book:
Robinson JP, Darzynkiewicz Z, Dressler LG, et al., editors. Current Protocols in Cytometry. New York (NY): John Wiley & Sons; 2001.
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