Divya Nair is currently enrolled for Ph.D under Dr Sudarslal S, Associate Professor, at School of Biotechnology.
Oxidative stress refers to the physiological stress caused by an imbalance between the systemic production of reactive oxygen species and the biological system's ability to detoxify the reactive intermediates thus leading to a number of diseases. Usually, the impact of oxidative stress on a cell is measured by analysing the oxidation states of molecules like glutathione. Carbonylation of proteins, one of the major oxidative stress induced modifications, can be considered as potential biomarkers for measuring the level of oxidative stresses. Recent investigations has revealed that carbonylation of proteins occur primarily at amino acid residues, proline, arginine, lysine, threonine etc. As part of the on-going studies, we aim at characterizing the carbonylated proteins from various model systems undergoing oxidative stresses. We also aim at testing the efficacy of various natural products as potential anti-oxidants through MS based proteomics strategies.
“Mass spectrometry based proteomic characterization of carbonylated proteins as indicators of oxidative stress”. Principal Investigator: Dr Sudarslal S, Associate Professor School of Biotechnology
Collaborator: Dr Harikumaran Nair, Assistant Professor, Mahatma Gandhi University
Amrita Agilent Analytical Research Centre,
School of Biotechnology,
Amrita Vishwa Vidyapeetham,Amritapuri,
Clappana P.O, Kollam, Kerala, 690525
+91-476-280- 3119 / 3126
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D. Nair, Vanuopadath, M., Dr. Bipin G. Nair, Dr. Jayashree G., and Nair, S. Sadasivan, “Identification and characterization of a library of surfactins and fengycins from a marine endophytic Bacillus sp.”, Journal of basic microbiology, vol. 56, pp. 1159–1172, 2016.[Abstract]
An endophytic bacterial strain from a marine green alga,Ulva lactuca, was isolated and identified by 16S rRNA gene sequencing method. The bacterial isolate was found to secrete two major families of cyclic depsilipopeptides, surfactins, and fengycins. Sequencing of the isolated lipopeptides was carried out using the MSndata obtained from an electrospray ionization (ESI) ion trap mass spectrometer coupled to an HPLC system. The assigned sequences were confirmed by a chemical derivatization approach involving esterification followed by mass spectrometric analysis. Distinction of leucine residues from isoleucine was established through a combined electron transfer dissociation-collision-induced dissociation (ETD-CID) method. The fengycins described in this study were found to cause significant delay of growth of two plants,Vigna radiata(mung bean) andOryza sativa(rice). To the best of our knowledge, this is the first study describing identification and characterization of cyclic peptides from an endophyticBacillus sp. isolated from marine algae.More »»