Qualification: 
M.Tech, B-Tech
minshamg@ay.amrita.edu

Minsha M. G. currently serves as Research Coordinator at Amrita Centre for Advanced Research, School of Ayurveda, Amritapuri. She completed her Bachelor of Technology in Biotechnology and Biochemical Engineering from Sree Chithra Thirunal College of Engineering, Thiruvananthapuram (2012) and Master of Technology in Molecular Medicine from Amrita Centre for Nanosciences and Molecular Medicine, Kochi (2014). She worked as Junior Research Fellow in Amrita Centre for Nanosciences and Molecular Medicine for 9 months in the project titled 'Developing anti- EGFR mutants phage display system based on antibody fragments for detection and targeted delivery of drugs to cancer cells'. Department of Biotechnology, Govt.of India, Cancer biology area.

Minsha's key research areas of interest include

  • Molecular biology and Biochemistry techniques: Recombinant DNA technology, PCR, bacterial expression systems, cloning of genes into vectors, expression analysis, techniques including AGE,PAGE,SEM.
  • Cell biology: Basic Cell culture , MTT assay
  • Elecrospinning- Nano fibrous mat synthesis using rotating mandrel and stationary targets.

Publications

Publication Type: Journal Article

Year of Publication Title

2018

S. Kuttappan, Anitha, A., Minsha Mallika Gopi, Menon, P. M., Sivanarayanan, T. B., Dr. Lakshmi Sumitra, and Dr. Manitha B. Nair, “BMP2 Expressing Genetically Engineered Mesenchymal Stem Cells on Composite Fibrous Scaffolds for Enhanced bone Regeneration in Segmental Defects”, Materials Science and Engineering: C, vol. 85, pp. 239 - 248, 2018.[Abstract]


The treatment of critical sized bone defect remains a significant challenge in orthopedics. The objective of the study is to evaluate the effect of the combination of bone morphogenetic protein 2 (BMP2) expressing genetically engineered mesenchymal stem cells (MSCs) [MSCs engineered using a multimam vector, pAceMam1, an emerging gene delivery vector] and an osteoconductive scaffold [silica coated nanohydroxyapatite-gelatin reinforced with fibers] in enhancing bone regeneration in critical sized segmental defects. The scaffold with transfected MSCs showed significantly higher viability, proliferation and osteogenic differentiation in vitro. Further, this group augmented union and new bone formation in critical sized rat femoral segmental defect at 12 weeks when compared to control groups (scaffold with MSCs and scaffold alone). These data demonstrated that the MSCs engineered for transient expression of BMP2 can improve the repair of segmental defects, which paves an avenue for using pAceMam1 as a vector for bone tissue regeneration.

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2016

R. Anney Matthew, Minsha Mallika Gopi, Menon, P., Dr. Jayakumar Rangasamy, and Dr. Lakshmi Sumitra, “Synthesis of Electrospun Silica Nanofibers for Protein/DNA Binding”, Materials Letters, vol. 184, pp. 5 - 8, 2016.[Abstract]


Silica is widely used as the nanomaterial carrier for DNA or protein delivery because of ease and multitude of methods for its synthesis and relatively simple ways with which its surface chemistry can be modified. In the present study, electrospun nano scale silica fibrous mats with diameters in the range of 250–320nm were synthesized using TEOS (Tetra ethyl ortho silicate) as a silica precursor along with Poly (vinyl pyrolidine) (PVP). These fibrous mats were used for effective binding/elution of plasmid DNA and BSA (Bovine Serum Albumin) under optimal conditions, which were demonstrated utilizing this combination of electrospun silica precursor and PVP. These silica fiberous mats are easier to control than silica nanoparticles and require less hazardous preparation than nanosheets developed via etching. The developed nanosilica mats could be a cost effective tool for DNA and protein delivery for different biotechnological and medical applications

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