MSc, BSc

Ms Divya Nedungadi P after completing her M.Sc in Biotechnology from Amrita School of Biotechnology in 2012 joined us as a PhD Scholar in the Cancer Biology Lab. She is currently working in a Department of Science and Technology (DST) funded project titled “Paraptosis- A newer approach to target cancer” under Dr Nandita Mishra, Assistant Professor.


  • MSc. Biotechnology, Amrita Vishwa Vidyapeetham (2012)
  • BSc. Biotechnology, PSG College of Arts & Science, Coimbatore (2010)


  • Award of Excellence from Amrita Vishwa Vidyapeetham,2013
  • Qualified ICMR-Junior Research Fellowship, 2012
  • Qualified GATE exam, 2012

Research experience

  • “Role of calpain and caspase-3 in regulating the mitochondrial fusion protein,Opa-1 during apoptosis”, Rajiv Gandhi Centre for Biotechnology, Kerala under Dr TR Santhoshkumar (Jan 2012-May 2012).
  • Protein purification at MIMS Research Foundation, Kerala under Dr R.V. Thampan (June 2011).
  • Recombinant DNA Technology and PCR, Shreedhar Bhatt Laboratory, Bangalore (May 2009)


Publication Type: Journal Article

Year of Publication Title


Anupama Binoy, Nedungadi, D., Katiyar, N., Chinchu Bose, Dr. Sahadev Shankarappa, Dr. Bipin G. Nair, and Dr. Nandita Mishra, “Plumbagin induces paraptosis in cancer cells by disrupting the sulfhydryl homeostasis and proteasomal function”, Chemico-Biological Interactions, p. 108733, 2019.[Abstract]

Plumbagin (PLB) is an active secondary metabolite extracted from the roots of Plumbago rosea. In this study, we report that plumbagin effectively induces paraptosis by triggering extensive cytoplasmic vacuolation followed by cell death in triple negative breast cancer cells (MDA-MB-231), cervical cancer cells (HeLa) and non-small lung cancer cells (A549) but not in normal lung fibroblast cells (WI-38). The vacuoles originated from the dilation of the endoplasmic reticulum (ER) and were found to be empty. The cell death induced by plumbagin was neither apoptotic nor autophagic. Plumbagin induced ER stress mainly by inhibiting the chymotrypsin-like activity of 26S proteasome as also evident from the accumulation of polyubiquitinated proteins. The vacuolation and cell death were found to be independent of reactive oxygen species generation but was effectively inhibited by thiol antioxidant suggesting that plumbagin could modify the sulfur homeostasis in the cellular milieu. Plumbagin also resulted in a decrease in mitochondrial membrane potential eventually decreasing the ATP production. This is the first study to show that Plumbagin induces paraptosis through proteasome inhibition and disruption of sulfhydryl homeostasis and thus further opens up the lead molecule to potential therapeutic strategies for apoptosis-resistant cancers.

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N. Velusamy, Anupama Binoy, Bobba, K. Naidu, Nedungadi, D., Dr. Nandita Mishra, and Bhuniya, S., “A bioorthogonal fluorescent probe for mitochondrial hydrogen sulfide: new strategy for cancer cell labeling”, Chem Commun (Camb)., vol. 53, no. 62, pp. 8802-8805, 2017.[Abstract]

We report the application of a chemodosimeter {'}turn on{'} fluorescent probe for detecting endogenous H2S formation in cancer cells. Mito-HS showed a bathochromic shift in the UV-vis-absorption spectrum from 355 nm to 395 nm in the presence of H2S. Furthermore{,} it showed an [similar]43-fold fluorescence enhancement at [small lambda]em = 450 nm in the presence of H2S (200 [small mu ]M). The cancer cell-specific fluorescence imaging reveals that Mito-HS has the ability to distinguish cancer cells from normal cells based on the level of endogenous H2S formation. In due course{,} Mito-HS would be a powerful cancer biomarker based on its ability to estimate endogenous H2S formation in living cells.

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Y. K. Yasoda, Bobba, K. Naidu, Nedungadi, D., Dutta, D., M. Kumar, S., Kothurkar, N., Dr. Nandita Mishra, and Bhuniya, S., “GSH-responsive biotinylated poly(vinyl alcohol)-grafted GO as a nanocarrier for targeted delivery of camptothecin”, RSC Adv., vol. 6, pp. 62385-62389, 2016.[Abstract]

A water-soluble and biocompatible polymer{,} i.e. biotinylated poly(vinyl alcohol)-grafted graphene oxide (GO){,} was used as a nanocarrier for targeted delivery of anticancer drug camptothecin (CPT). The extent of CPT release in the presence of glutathione (GSH) from GO-biotinPVA-CPT was monitored by the increase in the fluorescence intensity{,} at [small lambda]max = 450 nm. The cell-specific (HeLa) antiproliferative activity of GO-biotinPVA-CPT makes it suitable to be used for targeted delivery of chemotherapeutics to cancerous cells. More »»

Publication Type: Patent

Year of Publication Title


S. Bhuniya, Mishra, N., Velusamy, N., Anupama Binoy, Bobba, K. Naidu, and Nedungadi, D., “Flourescent Exomarker Probes for Hydrogen Sulfide Detection”, U.S. Patent 15 / 956 , 4742018.[Abstract]

A fluorescence probe with mitochondrial targeting and two-photon property, its preparation method and application in detecting and tracking endogenous H2S in samples or living cells. The fluorescent probe is prepared by a four-step preparation method and demonstrates a UV-vis absorption increment λab=395 nm and ˜43 fold higher fluorescence intensity in the presence of H2S. The probe further demonstrates stability, selectivity for H2S over competing agents and sensitivity as low as 20 nm. A method of detecting endogenous H2S rapidly in the absence of any external stimulators is provided. Samples are contacted with the probe and the changes in fluorescence are monitored to detect H2S levels. The disclosed probe is non-toxic and suitable as a biomarker and therapeutic molecule in cancer and other diseases.

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Publication Type: Conference Proceedings

Year of Publication Title


Dr. Nandita Mishra, Dr. Sanjay Pal, Nair, R. R., Krishna, A., Ajith, A., V, A., KS, D., Nedungadi, D., and P, C., “Expression and refolding of recombinant staphylococcal amidases in E. coli”, Proceedings of International Conference on Biotechnology for Innovative Applications (Amrita BioQuest 2013). Elsevier Publications, p. 106, 2013.

Faculty Research Interest: