Qualification: 
MSc, BSc
​muralidharanv@am.amrita.edu

Muralidharan currently serves as Junior Research Fellow at Amrita School of Biotechnology. He also serves as the technology assistant at Amrita Agilent Analytical Research Centre, School of Biotechnology. He received his masters in Biotechnology from Amrita Vishwa Vidyapeetham, Amritapuri in 2012. He is currently pursuing his doctoral degree in Biotechnology at Amrita Agilent Analytical Research Centre, School of Biotechnology.

ALMA MATER

  • MSc. Biotechnology, Amrita Vishwa Vidyapeetham (2012).
  • Diploma in Bioinstrumentation, Bharathiar University (2010).
  • Bsc. Biotechnology, Bharathiar University (2010).
     

EARLIER AFFILIATION

  • Technology Assistant, Amrita Agilent Analytical Research Centre (current).
  • Junior Research Fellow, Amrita Institute of Medical Sciences and Research Centre.
     

AWARDS / HONOR

  • Awarded with Junior Research Fellowship from Amrita Vishwa Vidyapeetham.
  • Qualified GATE-Biotechnology, March 2012.
     

RESEARCH INTERESTS AND EXPERTISE

  • Diabetes and Vascular Inflammation
  • Proteomics
  • Mass Spectrometry and High Performance Liquid Chromatography (HPLC) Techniques
     

TRAININGS AND WORKSHOPS

  • Two month operator's training course for Agilent LC/MS: Agilent OpenLAB Chemstation, Agilent 1290 uHPLC, Agilent 6340 LC/MS Iontrap, Agilent 8354 UV-Visible Spectrometer, held at the Amrita Agilent Analytical Research Centre, Amrita School of Biotechnology from 5th August - 5th October,2013 headed by Dr.Walter Schrenk, Associate Professor, Amrita School of Biotechnology.
  • Development of Analytical methods in High Performance Chromatography, uHPLC, 2-Dimensional HPLC and Mass Spectrometry for the analysis, isolation and identification of active components out of plant extracts with potential medical uses under the guidance of Dr.Walter Schrenk and Dr.Sudarslal S, the Associate Professors at Amrita School of Biotechnology, from January to May 2014.
     

RESEARCH PROJECTS

CONTACT DETAILS

Muralidharan V.
Amrita Agilent Analytical Research Centre
School of Biotechnology
Amrita Vishwa Vidyapeetham (Amritapuri Campus)
Clappana P.O., Kollam, Kerala, India. 690525
Tel +91 (0)476-2803000 Extn:3126

Publications

Publication Type: Journal Article

Year of Conference Publication Type Title

2016

Journal Article

D. Nair, Vanuopadath, M., Dr. Bipin G. Nair, Dr. Jayashree G., and Nair, S. Sadasivan, “Identification and characterization of a library of surfactins and fengycins from a marine endophytic Bacillus sp.”, Journal of basic microbiology, vol. 56, pp. 1159–1172, 2016.[Abstract]


An endophytic bacterial strain from a marine green alga,Ulva lactuca, was isolated and identified by 16S rRNA gene sequencing method. The bacterial isolate was found to secrete two major families of cyclic depsilipopeptides, surfactins, and fengycins. Sequencing of the isolated lipopeptides was carried out using the MSndata obtained from an electrospray ionization (ESI) ion trap mass spectrometer coupled to an HPLC system. The assigned sequences were confirmed by a chemical derivatization approach involving esterification followed by mass spectrometric analysis. Distinction of leucine residues from isoleucine was established through a combined electron transfer dissociation-collision-induced dissociation (ETD-CID) method. The fengycins described in this study were found to cause significant delay of growth of two plants,Vigna radiata(mung bean) andOryza sativa(rice). To the best of our knowledge, this is the first study describing identification and characterization of cyclic peptides from an endophyticBacillus sp. isolated from marine algae.

More »»

2016

Journal Article

M. Vanuopadath, Nair, D., Dr. Bipin G. Nair, and Nair, S. Sadasivan, “Post-translational Modifications of Proteins: Biomarkers and Therapeutic Targets for Diabetes Related Complications”, Current Proteomics, 2016, vol. 13, 2016.[Abstract]


Diabetes Mellitus is a chronic metabolic disorder that is often associated with various complications including micro- and macro- angiopathies. The increase in mortality rates associated with these complications is one among the major factors that compels to define proper therapeutic strategies. Protein signatures including their post-translational modifications that are modulated under various disease conditions enabled the biomarker discovery processes and thereby facilitate to predict and diagnose the disease onset at an earlier stage. The identification of the post-translationally modified proteins not only enabled the biomarker prediction associated with various disease conditions but also has a great impact in target based drug discovery process. Mass spectrometry based proteome profiling aided the identification and characterization of these site specific modifications that had a huge impact on various functional aspects in a biological context. In this review, we tried to compile and discuss the information available regarding some of the modified proteins with regard to deregulated glucose metabolism. Several commonly observed modifications including glycosylation, hydroxylation, phosphorylation, nitration, nitrosylation and carbonylation that seem to have a major role in the management of diabetes disorders are summarized. Moreover, the importance and impact of less explored post-translational modifications such as palmitoylation, carbamylation, deamidation and SUMOylation that are associated to diabetes related complications are described. More »»

2016

Journal Article

P. Malvi, Chaube, B., Singh, S. Vikram, Mohammad, N., Pandey, V., Vijayakumar, M. Vavachan, Radhakrishnan, R. Meenatheri, Vanuopadath, M., Nair, S. Sadasivan, Dr. Bipin G. Nair, and Bhat, M. Kumar, “Weight control interventions improve therapeutic efficacy of dacarbazine in melanoma by reversing obesity-induced drug resistance.”, Cancer Metab, vol. 4, p. 21, 2016.[Abstract]


BACKGROUND: Obesity-related cellular, metabolic, and molecular alterations have been shown to increase cancer risk and tumor progression and are associated with poorer therapeutic outcome in cancer patients. However, the impact of obesity and weight-control interventions on the therapeutic response in melanoma is poorly understood.
METHODS: High fat diet (HFD)-induced obese mouse model was used in this study to evaluate the outcome of dacarbazine (DTIC) therapy in melanoma. We employed LC-MS/MS to determine the quantity of the drug in tumor, and in various tissues. Unique in vitro approach was used to complement in vivo findings by culturing melanoma cells in either conditioned medium (CM) obtained from differentiated adipocytes or in serum collected from experimental mice.
RESULTS: We report that diet-induced obesity impairs the outcome of DTIC therapy and reduces overall survival in tumor-bearing mice. We provide evidence that obesity restricts the accessibility of DTIC to tumor tissue. Critically, upon curtailing adiposity, accumulation and efficacy of DTIC is significantly improved. Moreover, using appropriate in vitro approaches, we show that melanoma cells exhibit a drug-resistant phenotype when cultured in serum collected from diet-induced obese mice or in CM collected from 3T3-L1 adipocytes. The impaired therapeutic response to DTIC in obese state is mediated by fatty acid synthase (FASN), caveolin-1 (Cav-1), and P-glycoprotein (P-gp). The response to DTIC and overall survival were improved upon employing weight control interventions in the tumor-bearing HFD-fed (obese) mice.

CONCLUSIONS: This study indicates that obesity not only supports rapid melanoma progression but also impairs the outcome of chemotherapy, which can be improved upon employing weight control interventions. From clinically relevant point of view, our study exemplifies the importance of lifestyle interventions in the treatment of obesity-promoted cancers.

More »»
PDF iconweight-control-interventions-improve-therapeutic-efficacy-of-dacarbazine-in-melanoma-by-reversing-obesity-induced-drug-resistance-2016.pdf

2016

Journal Article

R. Biswas, Chatterjee, M., D'Morris, S., Baranwal, G., Warrier, S., Vasudevan, A., Vanuopadath, M., Nai, S., and Mohan, C., “Lactobacillus Fermentum Secreted Phenyl Lactic Acid Mediates Quorum Quenching in Pseudomonas Aeruginosa”, 2016.

2015

Journal Article

N. Mohammad, Singh, S. Vikram, Malvi, P., Chaube, B., Athavale, D., Vanuopadath, M., Nair, S. Sadasivan, Dr. Bipin G. Nair, and Bhat, M. Kumar, “Strategy to enhance efficacy of doxorubicin in solid tumor cells by methyl-β-cyclodextrin: Involvement of p53 and Fas receptor ligand complex”, Scientific reports, vol. 5, 2015.[Abstract]


Doxorubicin (DOX) is one of the preferred drugs for treating breast and liver cancers. However, its clinical application is limited due to severe side effects and the accompanying drug resistance. In this context, we investigated the effect on therapeutic efficacy of DOX by cholesterol depleting agent methyl-β-cyclodextrin (MCD), and explored the involvement of p53. MCD sensitizes MCF-7 and Hepa1-6 cells to DOX, Combination of MCD and marginal dose of DOX reduces the cell viability, and promoted apoptosis through induction of pro-apoptotic protein, Bax, activation of caspase-8 and caspase-7, down regulation of anti-apoptotic protein Bcl-2 and finally promoting PARP cleavage. Mechanistically, sensitization to DOX by MCD was due to the induction of FasR/FasL pathway through p53 activation. Furthermore, inhibition of p53 by pharmacological inhibitor pifithrin-α (PFT-α) or its specific siRNA attenuated p53 function and down-regulated FasR/FasL, thereby preventing cell death. Animal experiments were performed using C57BL/6J mouse isografted with Hepa1-6 cells. Tumor growth was retarded and survival increased in mice administered MCD together with DOX to as compared to either agent alone. Collectively, these results suggest that MCD enhances the sensitivity to DOX for which wild type p53 is an important determinant.

More »»
PDF iconstrategy-enhance-efficacy-doxorubicin-solid-tumor-cells-methyl-cyclodextrin-involvement-of-p-53-and-fas-receptor-ligand-complex-01july2015.pdf

Publication Type: Conference Proceedings

Year of Conference Publication Type Title

2014

Conference Proceedings

D. Nair, Vanuopadath, M., Dr. Bipin G. Nair, G, J., and S, S., “Isolation and Characterisation of Lipopeptides from a Bacillus subtilis sp. Strain, a marime algal endophyte”, Fourth International Seminar on Sustainable Utilisation of Tropical Plant Biomass- Ayur informatics. pp. 89-91, 2014.

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