Qualification: 
MPhil, MSc, BSc
anupama@am.amrita.edu

Anupama currently serves as Junior Research Fellow at Amrita School of Biotechnology under Department of Science and Technology (DST) funded project titled “Paraptosis- A newer approach to target cancer” under DrNandita Mishra, Assistant Professor. 

ALMA MATER

  • M.Phil. Biotechnology,Lovely Professional University (2010)
  • MSc. Biochemistry,Periyar University (2007)
  • BSc. ,Punjab University (2005)

EARLIER AFFILIATION

  • Junior Research Fellow at Amrita School of Biotechnology (current).
  • Teaching Assistant at Amrita School of Biotechnology (2012)

AWARDS / HONOR

  • Award of Excellence from Amrita Vishwa Vidyapeetham,2014
  • Qualified UGC-Junior Research Fellowship, 2012
  • Awarded University Academic Honour for standing First in Order of merit in M.Phil.2010

Research experience

Purification and characterisation of Protease from Clostridium species in Anaerobic       Digester:Insights into acidic and thermal stability of Protease from Central leather Research Institute, Chennai (2005)
Biotechnological innovations for the production of pectinases and its thermostabilisation (2010)

Research Interest

  • Cancer Biology
  • Protein Purification

Publications

Publication Type: Journal Article

Year of Publication Title

2020

S. Khatun, Biswas, S., Anupama Binoy, Podder, A., Dr. Nandita Mishra, and Bhuniya, S., “Highly chemoselective turn-on fluorescent probe for ferrous (Fe2+) ion detection in cosmetics and live cells”, Journal of Photochemistry and Photobiology B: Biology, vol. 209, p. 111943, 2020.[Abstract]


In modern society, the use of cosmetics has increased extensively; unfortunately, so-called several toxic metal salts are present as the colorant or filler in cosmetics. The ferrous ion (Fe2+) is one of the metal ions used in cosmetics as a colorant. Ferrous ion (Fe2+) is a vital component in live cells. Considering the adverse effect of high doses of ferrous ions in cosmetics and live cells, we developed a turn-on fluorescent probe PFe(II) for quantitative estimation of ferrous ion (Fe2+) in cosmetics and monitoring of labile ferrous (Fe2+) ion in live cells. The fluorescent probe PFe(II) showed a visual color change from colorless to orange in the presence of ferrous ion (Fe2+) in the cosmetics. We observed that UV-absorption increased at 390 nm upon incubation with ferrous ion (Fe2+). The probe PFe(II) has provided quantitative information on ferrous ion (Fe2+) in various cosmetics, kajol, lip balm, face foundation, mascara, eyeliner, lipliner, face makeup, sindoor, lipstick, nail polish in ppm level through the fluorescence signaling at 460 nm.The probe PFe(II) provided information on labile Fe2+ ion pool via a fluorescence imaging. It is a new addition to the diagnostic inventory for detecting ferrous ion in live cells and cosmetics.

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2019

Divya Nedungadi, Anupama Binoy, Vinod, V., Muralidharan Vanuopadath, Sudarslal Sadasivan Nair, Dr. Bipin G. Nair, and Dr. Nandita Mishra, “Ginger extract activates caspase independent paraptosis in cancer cells via ER stress, mitochondrial dysfunction, AIF translocation and DNA damage”, Nutrition and Cancer, pp. 1-13, 2019.[Abstract]


AbstractThe rhizome of ginger (Zingiber officinale) a common culinary agent is also known for its medicinal activity. We have earlier reported that pure 6-shogaol, an important component of ginger induces paraptosis in triple negative breast cancer (MDA-MB-231) and non small cell lung (A549) cancer cells. However, the chemopreventive potential of the whole ginger extract in food remains to be elucidated. Here, we demonstrate for the first time that ginger extract (GE) triggers similar anticancer activity/paraptosis against the same cell lines but through different molecular mechanisms. Q-TOF LC-MS analysis of the extract showed the presence of several other metabolites along with 6-shogaol and 6-gingerol. GE induces cytoplasmic vacuolation through ER stress and dilation of the ER. Drastic decrease in the mitochondrial membrane potential and ATP production along with the excess generation of ROS contributed to mitochondrial dysfunction. Consequently, GE caused the translocation of apoptosis inducing factor to the nucleus leading to the fragmentation of DNA. Taken together, these show a novel mechanism for ginger extract induced cancer cell death that can be of potential interest for cancer preventive strategies.

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2019

Anupama Binoy, Divya Nedungadi, Katiyar, N., Chinchu Bose, Dr. Sahadev Shankarappa, Dr. Bipin G. Nair, and Dr. Nandita Mishra, “Plumbagin induces paraptosis in cancer cells by disrupting the sulfhydryl homeostasis and proteasomal function”, Chemico-Biological Interactions, p. 108733, 2019.[Abstract]


Plumbagin (PLB) is an active secondary metabolite extracted from the roots of Plumbago rosea. In this study, we report that plumbagin effectively induces paraptosis by triggering extensive cytoplasmic vacuolation followed by cell death in triple negative breast cancer cells (MDA-MB-231), cervical cancer cells (HeLa) and non-small lung cancer cells (A549) but not in normal lung fibroblast cells (WI-38). The vacuoles originated from the dilation of the endoplasmic reticulum (ER) and were found to be empty. The cell death induced by plumbagin was neither apoptotic nor autophagic. Plumbagin induced ER stress mainly by inhibiting the chymotrypsin-like activity of 26S proteasome as also evident from the accumulation of polyubiquitinated proteins. The vacuolation and cell death were found to be independent of reactive oxygen species generation but was effectively inhibited by thiol antioxidant suggesting that plumbagin could modify the sulfur homeostasis in the cellular milieu. Plumbagin also resulted in a decrease in mitochondrial membrane potential eventually decreasing the ATP production. This is the first study to show that Plumbagin induces paraptosis through proteasome inhibition and disruption of sulfhydryl homeostasis and thus further opens up the lead molecule to potential therapeutic strategies for apoptosis-resistant cancers.

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2019

K. Naidu Bobba, Anupama Binoy, Koo, S., Nedungadi, D., Podder, A., Sharma, A., Dr. Nandita Mishra, Kim, J. Seung, and Bhuniya, S., “Direct readout protonophore induced selective uncoupling and dysfunction of individual mitochondria within cancer cells”, Chemical Communications, vol. 55, no. 45, pp. 6429-6432, 2019.[Abstract]


Concurrently, manipulation of mitochondrial activity and its monitoring have enormous significance in cancer therapy and diagnosis. In this context, a fluorescent probe MitoDP has been developed for validating H2S mediated protonophore (2,4-dinitrophenol, DNP) induced mitochondrial membrane potential change, ROS formation and ATP depletion in cancer cells. The extent of protonophore activation for mitochondrial dysfunction is monitored through fluorescence signalling at 450 nm. The current study provides a proof for the concept of endogenous H2S-mediated controlled and spatial release of bioactive agents, or toxins specifically in mitochondria of cancer cells.

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2018

P. Sagitha, Reshmi, C. R., Sundaran, S. P., Anupama Binoy, Dr. Nandita Mishra, and Sujith, A., “In-vitro evaluation on drug release kinetics and antibacterial activity of dextran modified polyurethane fibrous membrane.”, International Journal of Biological Macromolecules, vol. 126, pp. 717-730, 2018.[Abstract]


pH stimuli drug release nanofibrous membranes of polyurethane/dextran were developed for tailoring of antibacterial wound dressings. Incorporation of dextran in polyurethane (PU) showed increment in hydrophilicity, vapour transmission rate, percentage sorption values, and biodegradability. Dextran also acts as reinforcement filler in PU matrix. Dextran induces a high degree of platelet adhesion and hemostasis potential which is essential for promoting the wound healing process. Moreover, 20 wt% dextran loaded membranes (PU/20D) exhibited enhanced cell proliferation, attachment and viability against 3T3 fibroblasts. Curcumin loaded PU/20 dextran membrane exhibited pH-controlled drug release potency and synergistic antibacterial activity against gram-positive bacteria. It is confirmed that, PU/20D membranes could promote, pH-controlled drug release and synergistic antibacterial activity for a promising wound dressing material.

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2017

N. Velusamy, Anupama Binoy, Bobba, K. Naidu, Divya Nedungadi, Dr. Nandita Mishra, and Bhuniya, S., “A bioorthogonal fluorescent probe for mitochondrial hydrogen sulfide: new strategy for cancer cell labeling”, Chem Commun (Camb)., vol. 53, no. 62, pp. 8802-8805, 2017.[Abstract]


We report the application of a chemodosimeter {'}turn on{'} fluorescent probe for detecting endogenous H2S formation in cancer cells. Mito-HS showed a bathochromic shift in the UV-vis-absorption spectrum from 355 nm to 395 nm in the presence of H2S. Furthermore{,} it showed an [similar]43-fold fluorescence enhancement at [small lambda]em = 450 nm in the presence of H2S (200 [small mu ]M). The cancer cell-specific fluorescence imaging reveals that Mito-HS has the ability to distinguish cancer cells from normal cells based on the level of endogenous H2S formation. In due course{,} Mito-HS would be a powerful cancer biomarker based on its ability to estimate endogenous H2S formation in living cells.

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2017

C. R. Reshmi, Menon, T., Anupama Binoy, Dr. Nandita Mishra, Elyas, K. K., and Sujith, A., “Poly(L-lactide-co-caprolactone)/collagen electrospun mat: Potential for wound dressing and controlled drug delivery”, International Journal of Polymeric Materials and Polymeric Biomaterials, vol. 66, no. 13, pp. 645-657, 2017.[Abstract]


Here we report a novel bioactive electrospun mat based on poly(L-lactide-co-caprolactone) (PLLC) and collagen for wound dressing and sustained drug delivery of gentamicin. PLLC/collagen electrospun mat loaded with 10% gentamicin showed bioactivity for 15 days against Gram-positive and Gram-negative bacteria. The in vitro cell culture of 3T3 fibroblasts confirmed that these electrospun mat provide an increased specific interface area and hydrophilicity to enhance cell attachment, proliferation, and migration. The modified PLLC/collagen mat provided an excellent enhancement in properties of antibacterial wound dressings with a minimum in vitro toxicity and high potency for promoting wound healing stages. © 2017 Taylor & Francis.

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Publication Type: Conference Proceedings

Year of Publication Title

2020

Anupama Binoy, Divya Nedungadi, Damu Sunilkumar, Dr. Bipin G. Nair, and Dr. Nandita Mishra, “Role of AIF in Plumbagin induced Paraptosis-A caspase independent cell death in MDA-MB-231 cancer cells”, 39th Annual Conference of Indian Association for Cancer Research (IACR-2020): “Leading the Fight against Cancer”, Rajiv Gandhi Centre for Biotechnology (RGCB), Trivandrum, Kerala, India, February 5-7. 2020.

2017

Divya Nedungadi, Megha Prasad, Anupama Binoy, Patra, G., Silva, J. D., Chatterjee, M., Jacob, A., Babu, A. S., Alexin, R., Dr. Bipin G. Nair, Dr. Sanjay Pal, and Dr. Nandita Mishra, “Anthelmintic properties of ginger compounds”, 27th Swadeshi Science Congress, November 7-9, 2017. Kerala, India, 2017.

Publication Type: Patent

Year of Publication Title

2018

S. Bhuniya, Dr. Nandita Mishra, Velusamy, N., Anupama Binoy, Bobba, K. Naidu, and Divya Nedungadi, “Flourescent Exomarker Probes for Hydrogen Sulfide Detection”, U.S. Patent 15 / 956 , 4742018.[Abstract]


A fluorescence probe with mitochondrial targeting and two-photon property, its preparation method and application in detecting and tracking endogenous H2S in samples or living cells. The fluorescent probe is prepared by a four-step preparation method and demonstrates a UV-vis absorption increment λab=395 nm and ˜43 fold higher fluorescence intensity in the presence of H2S. The probe further demonstrates stability, selectivity for H2S over competing agents and sensitivity as low as 20 nm. A method of detecting endogenous H2S rapidly in the absence of any external stimulators is provided. Samples are contacted with the probe and the changes in fluorescence are monitored to detect H2S levels. The disclosed probe is non-toxic and suitable as a biomarker and therapeutic molecule in cancer and other diseases.

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CONTACT

Anupama
School of Biotechnology
Amrita Vishwa Vidyapeetham (Amritapuri Campus)
Clapana P.O., Kollam, Kerala, India. 690525

Faculty Research Interest: