Qualification: 
MPhil, MSc, BSc
anupama@am.amrita.edu

Anupama currently serves as Junior Research Fellow at Amrita School of Biotechnology under Department of Science and Technology (DST) funded project titled “Paraptosis- A newer approach to target cancer” under DrNandita Mishra, Assistant Professor. 

ALMA MATER

  • M.Phil. Biotechnology,Lovely Professional University (2010)
  • MSc. Biochemistry,Periyar University (2007)
  • BSc. ,Punjab University (2005)

EARLIER AFFILIATION

  • Junior Research Fellow at Amrita School of Biotechnology (current).
  • Teaching Assistant at Amrita School of Biotechnology (2012)

AWARDS / HONOR

  • Award of Excellence from Amrita Vishwa Vidyapeetham,2014
  • Qualified UGC-Junior Research Fellowship, 2012
  • Awarded University Academic Honour for standing First in Order of merit in M.Phil.2010

Research experience

Purification and characterisation of Protease from Clostridium species in Anaerobic       Digester:Insights into acidic and thermal stability of Protease from Central leather Research Institute, Chennai (2005)
Biotechnological innovations for the production of pectinases and its thermostabilisation (2010)

Research Interest

  • Cancer Biology
  • Protein Purification

Publications

Publication Type: Journal Article

Year of Publication Publication Type Title

2018

Journal Article

P. Sagitha, Reshmi, C. R., Sundaran, S. P., Anupama Binoy, Dr. Nandita Mishra, and Sujith, A., “In-vitro evaluation on drug release kinetics and antibacterial activity of dextran modified polyurethane fibrous membrane.”, International Journal of Biological Macromolecules, vol. 126, pp. 717-730, 2018.[Abstract]


pH stimuli drug release nanofibrous membranes of polyurethane/dextran were developed for tailoring of antibacterial wound dressings. Incorporation of dextran in polyurethane (PU) showed increment in hydrophilicity, vapour transmission rate, percentage sorption values, and biodegradability. Dextran also acts as reinforcement filler in PU matrix. Dextran induces a high degree of platelet adhesion and hemostasis potential which is essential for promoting the wound healing process. Moreover, 20 wt% dextran loaded membranes (PU/20D) exhibited enhanced cell proliferation, attachment and viability against 3T3 fibroblasts. Curcumin loaded PU/20 dextran membrane exhibited pH-controlled drug release potency and synergistic antibacterial activity against gram-positive bacteria. It is confirmed that, PU/20D membranes could promote, pH-controlled drug release and synergistic antibacterial activity for a promising wound dressing material.

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2017

Journal Article

N. Velusamy, Anupama Binoy, Bobba, K. Naidu, Nedungadi, D., Dr. Nandita Mishra, and Bhuniya, S., “A bioorthogonal fluorescent probe for mitochondrial hydrogen sulfide: new strategy for cancer cell labeling”, Chem Commun (Camb)., vol. 53, no. 62, pp. 8802-8805, 2017.[Abstract]


We report the application of a chemodosimeter {'}turn on{'} fluorescent probe for detecting endogenous H2S formation in cancer cells. Mito-HS showed a bathochromic shift in the UV-vis-absorption spectrum from 355 nm to 395 nm in the presence of H2S. Furthermore{,} it showed an [similar]43-fold fluorescence enhancement at [small lambda]em = 450 nm in the presence of H2S (200 [small mu ]M). The cancer cell-specific fluorescence imaging reveals that Mito-HS has the ability to distinguish cancer cells from normal cells based on the level of endogenous H2S formation. In due course{,} Mito-HS would be a powerful cancer biomarker based on its ability to estimate endogenous H2S formation in living cells.

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2017

Journal Article

C. R. Reshmi, Menon, T., Anupama Binoy, Dr. Nandita Mishra, Elyas, K. K., and Sujith, A., “Poly(L-lactide-co-caprolactone)/collagen electrospun mat: Potential for wound dressing and controlled drug delivery”, International Journal of Polymeric Materials and Polymeric Biomaterials, vol. 66, no. 13, pp. 645-657, 2017.[Abstract]


Here we report a novel bioactive electrospun mat based on poly(L-lactide-co-caprolactone) (PLLC) and collagen for wound dressing and sustained drug delivery of gentamicin. PLLC/collagen electrospun mat loaded with 10% gentamicin showed bioactivity for 15 days against Gram-positive and Gram-negative bacteria. The in vitro cell culture of 3T3 fibroblasts confirmed that these electrospun mat provide an increased specific interface area and hydrophilicity to enhance cell attachment, proliferation, and migration. The modified PLLC/collagen mat provided an excellent enhancement in properties of antibacterial wound dressings with a minimum in vitro toxicity and high potency for promoting wound healing stages. © 2017 Taylor & Francis.

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CONTACT

Anupama
School of Biotechnology
Amrita Vishwa Vidyapeetham (Amritapuri Campus)
Clapana P.O., Kollam, Kerala, India. 690525

Faculty Research Interest: