Qualification: 
MSc
vidhyaprakash@am.amrita.edu

Vidhya Prakash currently serves as Assistant Professor at the School of Biotechnology.

She received her masters in Microbiology from Bharathiar University, Coimbatore in 2004.

Teaching

Extension Activities

Subject matter expert (SME) in field of Microbiology developing Sakshat Amrita Virtual Labs project which is an initiative of Ministry of Human Resource Development (MHRD) under National Mission on Education (NME) through Information and Communication Technology (ICT) in Amrita .

Subject matter expert in Biology for Online learning labs (O-labs), educational initiative pioneered by CREATE @ Amrita, in partnership with CDAC, Mumbai under a research grant from the Department of Information Technology,Government of India.

Research Interests

Publications

Publication Type: Journal Article

Year of Publication Title

2018

Vidhya Prakash, H. Sreetha, K. H. Poornima, K. N. Lakshmimol, R. Regma, H. Fathima, T. V. Vishnu, S. Venu, Dr. Bipin G. Nair, and Dr. Sanjay Pal, “Antagonistic Effects of Bacteriocins from Lactobacillus Spp. against Enteric Pathogens”, Pollution Research Paper, vol. 37, pp. 128-134, 2018.[Abstract]


Bacteriocins from lactic acid bacteria (LAB) are natural antimicrobial peptides or proteins with potential applications in food preservation and healthcare. The study aimed at screening and characterization of bacteriocins from the secretome of five Lactobacillus strains. We were able to screen the antagonistic potential of two strains, L. fermentum and L. casei 335 against the multidrug resistant pathogens, Escherichia coli MDR (multidrug resistant) and Salmonella typhi isolated from sewage. Different dialysate fractions of the Lactobacillus strains were screened by soft agar diffusion and microtitre inhibition assay. Dialysate fractions from ammonium sulfate precipitate, at 65% (LF 65) and at 90% (LF 90) from L. fermentum were tested. LF60 (40μg/mL) was found to be inhibitory to the growth of E. coli MDR strain. Minimum Inhibitory Concentrations (MIC) against biofilm formation were found to be 40μg/mL for both E. coli MDR and Salmonella typhi, though inhibition was found at sub MIC of 20 μg/mL. Biofilm dispersal inhibition of E. coli MDR and S. typhi demonstrated a percentage inhibition rate of 50%. For initial characterization of the proteome of LAB strains, Tricine SDS-PAGE was performed which yielded a protein band of molecular size of 11 kDa from LF 60 fractions. Gel overlay assay showed an inhibitory effect on growth of E. coli MDR, indicating the presence of bacteriocin activity. Caenorhabditis elegans was employed as a nematode model to study the in vivo protection against E. coli MDR. Significant enhanced survival (91%) of the nematode was observed on treatment with LF 60. The efficacy of these partially purified preparations in inhibiting enteric pathogens showcase a prospective strategy of application of these antimicrobials in food and other environmental applications where cost of purification is of concern.

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Publication Type: Conference Proceedings

Year of Publication Title

2017

Vidhya Prakash, “Partial Purification and Characterisation of Potential Bacteriocin Producers from Fermented Rice Water”, Proceedings of 27th Swadeshi Science Congress. 2017.

2016

Vidhya Prakash, “Studies on Antagonistic Effects of Partially Purified Bacteriocins from Microflora of Fermented Vegetables Against Enteric Pathogens”, NextGen Genomics, Biology, Bioinformatics and Technologies (NGBT) Conference. 2016.

2015

Vidhya Prakash, “Detection, Partial Purification and Characterization of Bacteriocins from Microflora of Pineapple Wine”, Kerala Science congress . 2015.

2015

Vidhya Prakash, “Effects of Nisin in Enhancing the Effect of Conventional Antibiotics against a Few Enteric Pathogens: An in Vitro Study”, Proceedings of International Conference on New Horizons in Biotechnology (NHBT-2015). 2015.

2013

Vidhya Prakash, “Studies on Biofilm Inhibition and Antibacterial Activity of Plant Extracts Against Dental Plaque Pathogens”, International Conference on Biotechnology for Innovative Applications Amrita Bioquest 2013. 2013.

2011

Vidhya Prakash, “Enhancement of Biodegradative Activity in Commercial and Lab Scale Compost Preparations with Lignocellulolytic Bacteria and Nitrogen Fixing Bacteria as Supplements - A Comparative Study”, Proceedings of International Conference on New Horizons in Biotechnology (NHBT-2011). 2011.

Publication Type: Conference Paper

Year of Publication Title

2013

Vidhya Prakash, ,, Surendran, S., Krishnan, A., Mohan, A., Nair, P., and Nair, B., “Studies on biofilm inhibition and antibacterial properties of plant extractsagainst Streptococcus mutans”, in Amrita Bioquest 2013, 2013.