Qualification: 
Ph.D, MSc, M.Tech
nanditamishra@am.amrita.edu

Dr. Nandita Mishra currently serves as Assistant Professor at Amrita School of Biotechnology.

Education

  • Ph.D. Biotechnology, Indian Institute of Technology, Kharagpur.
  • M.Tech. Biotechnology and Engineering, Indian Institute of Technology, Kharagpur.
  • M.Sc. Life Sciences, Sambalpur University, Orissa.
  • B.Sc. Botany (Honors) Sambalpur University, Orissa.
     

Research Interests

Awards and Scholarships

  • Awarded Fellowship from Govt. of India for Doctoral studies.
  • Ministry of Human Resources & Dev, Government of India fellowship for M. Tech. studies.
  • Qualified Graduate Aptitude Test in Engineering (GATE).
  • Visiting Research fellow, University of Guelph, Canada (2005) with Prof. Manish Raizada.
  • Postdoctoral fellowship, University of Texas Health Science Center at San Antonio, Texas, USA with Prof. Pothana Saikumar (2006-2010).
  • Awarded Scholarship from Government of Orissa for Excellence in Secondary Examination.
  • Award of Excellence from Amrita Vishwa Vidyapeetham (2012-13)

Teaching

  1. BIO317/Research Methodology/B.Sc. Biotechnology and B.Sc. Microbiology/2 Credits/Semester 5
  2. BIO399/ Project /B.Sc. Biotechnology and B.Sc. Microbiology/10 Credits/Semester 6
  3. BIO401/Molecular Biology/M.Sc. Biotechnology and M.Sc. Microbiology/3 Credits/Semester 1
  4. BIO497/Research Methodology/M.Sc. Biotechnology and M.Sc. Microbiology/2 Credits/Semester 1
  5. BIO408/Recombinant DNA Technology/M.Sc. Biotechnology and M.Sc. Microbiology/3  Credits/Semester 2
  6. BIO481/Recombinant DNA Technology Lab/M.Sc. Biotechnology and M.Sc. Microbiology/2  Credits/Semester 2
  7. BIO517/ Plant Biotechnology/ M.Sc. Biotechnology /3 Credits/ Semester 2
  8. BIO588/Cell & Molecular Biology Lab/M.Sc. Biotechnology & M.Sc. Microbiology/2  Credits/ Semester 3/
  9. BIO599/Project/M.Sc. Biotechnology & M.Sc. Microbiology/10 Credits/Semester 4
  10. BIO464/ Cancer Biology Elective/ MSc Biotechnology/MSc Microbiology/4 Credits/Semester 3
     

Grants received

Publications

Publication Type: Journal Article

Year of Conference Publication Type Title

2018

Journal Article

C. Porayath, Suresh, M. K., Biswas, R., Dr. Bipin G. Nair, Dr. Nandita Mishra, and Dr. Sanjay Pal, “Autolysin Mediated Adherence of Staphylococcus Aureus with Fibronectin, Gelatin and Heparin”, International Journal of Biological Macromolecules, 2018.

2018

Journal Article

D. Nedungadi, Binoy, A., Nanjan Pandurangan, Pal, S., Dr. Bipin G. Nair, and Dr. Nandita Mishra, “6-Shogaol Induces Caspase-independent Paraptosis in Cancer Cells Via Proteasomal Inhibition”, Exp Cell Res, vol. 364, no. 2, pp. 243-251, 2018.[Abstract]


An α, β-unsaturated carbonyl compound of ginger, 6-Shogaol (6S), induced extensive cytoplasmic vacuolation and cell death in breast cancer cell (MDA-MB-231) and non-small lung cancer (A549) cells. In the presence of autophagic inhibitors the cells continued to exhibit cytoplasmic vacuolation and cell death clearly distinguishing it from the classic autophagic process. 6S induced death did not exhibit the characteristic apoptotic features like caspase cleavage, phosphatidyl serine exposure and DNA fragmentation. The immunofluorescence with the Endoplasmic Reticulum (ER) resident protein, calreticulin indicated that the vacuoles were of ER origin, typical of paraptosis. This was supported by the increase in level of microtubule associated protein light chain 3B (LC3 I and LC3 II) and polyubiquitin binding protein, p62. The level of ER stress markers like polyubiquitinated proteins, Bip and CHOP also consistently increased. We have found that 6S inhibits the 26S proteasome. The proteasomal inhibitory activity was elucidated by a) molecular docking of 6S onto the active site of β5 subunit and b) reduced fluorescence by the fluorogenic substrate of the chymotrypsin-like subunit. In conclusion these studies demonstrate for the first time that proteasomal inhibition by 6S induces cell death via paraptosis. So 6-shogaol may act as a template for anti-cancer lead discovery against the apoptosis resistant cancer cells.

More »»

2017

Journal Article

C. R. Reshmi, Menon, T., Binoy, A., Dr. Nandita Mishra, Elyas, K. K., and Sujith, A., “Poly(L-lactide-co-caprolactone)/collagen electrospun mat: Potential for wound dressing and controlled drug delivery”, International Journal of Polymeric Materials and Polymeric Biomaterials, vol. 66, no. 13, pp. 645-657, 2017.[Abstract]


Here we report a novel bioactive electrospun mat based on poly(L-lactide-co-caprolactone) (PLLC) and collagen for wound dressing and sustained drug delivery of gentamicin. PLLC/collagen electrospun mat loaded with 10% gentamicin showed bioactivity for 15 days against Gram-positive and Gram-negative bacteria. The in vitro cell culture of 3T3 fibroblasts confirmed that these electrospun mat provide an increased specific interface area and hydrophilicity to enhance cell attachment, proliferation, and migration. The modified PLLC/collagen mat provided an excellent enhancement in properties of antibacterial wound dressings with a minimum in vitro toxicity and high potency for promoting wound healing stages. © 2017 Taylor & Francis.

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2017

Journal Article

N. Velusamy, Binoy, A., Bobba, K. Naidu, Nedungadi, D., Dr. Nandita Mishra, and Bhuniya, S., “A bioorthogonal fluorescent probe for mitochondrial hydrogen sulfide: new strategy for cancer cell labeling”, Chem Commun (Camb)., vol. 53, no. 62, pp. 8802-8805, 2017.[Abstract]


We report the application of a chemodosimeter {'}turn on{'} fluorescent probe for detecting endogenous H2S formation in cancer cells. Mito-HS showed a bathochromic shift in the UV-vis-absorption spectrum from 355 nm to 395 nm in the presence of H2S. Furthermore{,} it showed an [similar]43-fold fluorescence enhancement at [small lambda]em = 450 nm in the presence of H2S (200 [small mu ]M). The cancer cell-specific fluorescence imaging reveals that Mito-HS has the ability to distinguish cancer cells from normal cells based on the level of endogenous H2S formation. In due course{,} Mito-HS would be a powerful cancer biomarker based on its ability to estimate endogenous H2S formation in living cells.

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2016

Journal Article

Y. K. Yasoda, Bobba, K. Naidu, Nedungadi, D., Dutta, D., M. Kumar, S., Kothurkar, N., Dr. Nandita Mishra, and Bhuniya, S., “GSH-responsive biotinylated poly(vinyl alcohol)-grafted GO as a nanocarrier for targeted delivery of camptothecin”, RSC Adv., vol. 6, pp. 62385-62389, 2016.[Abstract]


A water-soluble and biocompatible polymer{,} i.e. biotinylated poly(vinyl alcohol)-grafted graphene oxide (GO){,} was used as a nanocarrier for targeted delivery of anticancer drug camptothecin (CPT). The extent of CPT release in the presence of glutathione (GSH) from GO-biotinPVA-CPT was monitored by the increase in the fluorescence intensity{,} at [small lambda]max = 450 nm. The cell-specific (HeLa) antiproliferative activity of GO-biotinPVA-CPT makes it suitable to be used for targeted delivery of chemotherapeutics to cancerous cells. More »»

2010

Journal Article

Dr. Nandita Mishra, Kar, R., Singha, P. K., Venkatachalam, M. A., McEwen, D. G., and Saikumar, P., “Inhibition of Mitochondrial Division Through Covalent Modification of Drp1 Protein by 15 deoxy-Delta(12,14)-prostaglandin J2”, Biochemical and biophysical research communications, vol. 395, pp. 17–24, 2010.[Abstract]


Arachidonic acid derived endogenous electrophile 15d-PGJ2 has gained much attention in recent years due to its potent anti-proliferative and anti-inflammatory actions mediated through thiol modification of cysteine residues in its target proteins. Here, we show that 15d-PGJ2 at 1µM concentration converts normal mitochondria into large elongated and interconnected mitochondria through direct binding to mitochondrial fission protein Drp1 and partial inhibition of its GTPase activity. Mitochondrial elongation induced by 15d-PGJ2 is accompanied by increased assembly of Drp1 into large oligomeric complexes through plausible intermolecular interactions. The role of decreased GTPase activity of Drp1 in the formation of large oligomeric complexes is evident when Drp1 is incubated with a non-cleavable GTP analog, GTPγS or by a mutation that inactivated GTPase activity of Drp1 (K38A). The mutation of cysteine residue (Cys644) in the GTPase effector domain, a reported target for modification by reactive electrophiles, to alanine mimicked K38A mutation induced Drp1 oligomerization and mitochondrial elongation, suggesting the importance of cysteine in GED to regulate the GTPase activity and mitochondrial morphology. Interestingly, treatment of K38A and C644A mutants with 15d-PGJ2 resulted in super oligomerization of both mutant Drp1s indicating that 15d-PGJ2 may further stabilize Drp1 oligomers formed by loss of GTPase activity through covalent modification of middle domain cysteine residues. The present study documents for the first time the regulation of a mitochondrial fission activity by a prostaglandin, which will provide clues for understanding the pathological and physiological consequences of accumulation of reactive electrophiles during oxidative stress, inflammation and degeneration.

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2010

Journal Article

R. Kar, Dr. Nandita Mishra, Singha, P. K., Venkatachalam, M. A., and Saikumar, P., “Mitochondrial Remodeling Following Fission Inhibition by 15d-PGJ2 Involves Molecular Changes in Mitochondrial Fusion Protein OPA1”, Biochemical and biophysical research communications, vol. 399, pp. 548–554, 2010.[Abstract]


We showed earlier that 15 deoxy Δ12, 14 prostaglandin J2 (15d-PGJ2) inactivates Drp1 and induces mitochondrial fusion [1]. However, prolonged incubation of cells with 15d-PGJ2 resulted in remodeling of fused mitochondria into large swollen mitochondria with irregular cristae structure. While initial fusion of mitochondria by 15d-PGJ2 required the presence of both outer (Mfn1 and Mfn2) and inner (OPA1) mitochondrial membrane fusion proteins, later mitochondrial changes involved increased degradation of the fusion protein OPA1 and ubiquitination of newly synthesized OPA1 along with decreased expression of Mfn1 and Mfn2, which likely contributed to the loss of tubular rigidity, disorganization of cristae, and formation of large swollen degenerated dysfunctional mitochondria. Similar to inhibition of Drp1 by 15d-PGJ2, decreased expression of fission protein Drp1 by siRNA also resulted in the loss of fusion proteins. Prevention of 15d-PGJ2 induced mitochondrial elongation by thiol antioxidants prevented not only loss of OPA1 isoforms but also its ubiquitination. These findings provide novel insights into unforeseen complexity of molecular events that modulate mitochondrial plasticity.

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2006

Journal Article

Dr. Nandita Mishra, Dr. Sanjay Pal, K, M. T., and S, D., “Production and characterization of arabinogalactan protein (AGP) from a hairy root line of Catharanthus roseus (L.) G. Don”, Indian Journal of Biotechnology, vol. 5, pp. 211-216, 2006.[Abstract]


Arabinogalactan protein (AGP), a class of cell wall proteoglycan, was isolated from the hairy root cultures of a newly developed hairy root line IIT-BT/D1 of Catharanthus roseus (L.) G. Don. AGP was found to be present both in the roots (0.3 mg/g fresh weight) and in the spent media (47 mg/L). The compositional analysis revealed the predominance of arabinose and galactose sugar, a characteristic feature of AGP. More »»

Publication Type: Conference Proceedings

Year of Conference Publication Type Title

2015

Conference Proceedings

D. Nedungadi, ,, Pandurangan Nanjan, Nair, B. G., Dr. Asoke Banerji, and Dr. Nandita Mishra, “Caspase Independent Cell Death in Malignant Breast Cancer Cells MDA MB-231 by Chalcone Derivatives”, XXXIX All India Cell Biology Conference,IISER. Trivandrum, India, 2015.

2013

Conference Proceedings

Dr. Nandita Mishra, Dr. Sanjay Pal, Nair, R. R., Krishna, A., Ajith, A., V, A., KS, D., Nedungadi, D., and P, C., “Expression and refolding of recombinant staphylococcal amidases in E. coli”, Proceedings of International Conference on Biotechnology for Innovative Applications (Amrita BioQuest 2013). Elsevier Publications, p. 106, 2013.

2011

Conference Proceedings

Dr. Nandita Mishra, Kar, R., Singha, P. K., Venkatachalam, M. A., and Saikumar, P., “Ethacrynic acid induced mitochondrial fusion is mediated by Inhibition of fission protein Drp1”, XXXV All India Cell Biology Conference,16th – 18th Dec . NISER, Bhubaneswar, India, 2011.

2010

Conference Proceedings

P. K. Singha, Dr. Nandita Mishra, Kar, R., Pandeswara, S., Venkatachalam, M. A., and Saikumar, P., “Targeting LC3-SQSTM1 (p62) signaling axis to treat apoptosis resistant and metastatic breast cancers”, American Association for Cancer Research Annual Meeting, April 17-21. Washington DC , 2010.

2009

Conference Proceedings

Dr. Nandita Mishra, R, K., PK, S., MA, V., and P, S., “Inhibition of Drp1 disassembly on mitochondrial remodeling”, Keystone Symposium, March 22-27. Whistler, Canada, 2009.

2008

Conference Proceedings

Dr. Nandita Mishra, R, K., PK, S., MA, V., and P, S., “Mitochondrial Fusion Precedes Cell Death Induced by 15-Deoxy Δ12, 14 Prostaglandin J2”, The American Society for Cell Biology Conference . San Francisco , 2008.

2004

Conference Proceedings

Dr. Nandita Mishra and S, D., “Metabolite profiling in hairy root cultures of Catharanthus roseus”, IUPAC International Conference on Biodiversity and Natural products:Chemistry and Medical Applications, Jan. 26-31. New Delhi, 2004.

2003

Conference Proceedings

Dr. Nandita Mishra, B, C., W, B., and S, D., “Arabinogalactan proteins from hairy root cultures of Catharanthus roseus”, 51st Annual Congress of the Society for Medicinal Plant Research, August 31st-Sep 4th. Kiel, Germany, 2003.

2001

Conference Proceedings

Dr. Nandita Mishra and S, D., “Hairy root cultures of Catharanthus roseus: Production of important alkaloids”, XXV All India Cell Biology Conference, Nov 1-3. IISc, Bangalore, India, 2001.

Publication Type: Conference Paper

Year of Conference Publication Type Title

2013

Conference Paper

Dr. Nandita Mishra, Suresh, A., S, A., P.V, A., .P, P., O.J, S., P, C., and Dr. Sanjay Pal, “Studies on Probiotic Strains from Fermented Foods & Beverages in Kerala”, in Proceedings of International Conference on Biotechnology for Innovative Applications (Amrita BioQuest 2013), 2013.

2013

Conference Paper

R. M. Nair, Nambiar, S. S., TV, V., L, V. Prabhu, Menon, D., Gandhi, B. Balan, P, C., Dr. Nandita Mishra, and Dr. Sanjay Pal, “Characterization of Fibronectin Isoforms and Fragments”, in Proceedings of International Conference on Biotechnology for Innovative Applications (Amrita BioQuest 2013), 2013.

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