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Publication Type : Journal Article
Thematic Areas : Advanced Materials and Green Technologies
Publisher : Chem. Commun
Source : Chemical Communications
Campus : Amritapuri, Coimbatore
School : School of Biotechnology, School of Engineering
Center : Center for Excellence in Advanced Materials and Green Technologies, Center for Industrial Research and Innovation (ACIRI)
Department : biotechnology, Civil
Year : 2019
Abstract : Concurrently, manipulation of mitochondrial activity and its monitoring have enormous significance in cancer therapy and diagnosis. In this context, a fluorescent probe MitoDP has been developed for validating H2S mediated protonophore (2,4-dinitrophenol, DNP) induced mitochondrial membrane potential change, ROS formation and ATP depletion in cancer cells. The extent of protonophore activation for mitochondrial dysfunction is monitored through fluorescence signalling at 450 nm. The current study provides a proof for the concept of endogenous H2S-mediated controlled and spatial release of bioactive agents, or toxins specifically in mitochondria of cancer cells.
Cite this Research Publication : Bobba KN, Binoy A, Koo S, Nedungadi D, Podder A, Sharma A, Mishra N*, Kim JS*, Bhuniya S* Direct readout protonophore induced selective uncoupling and dysfunction of individual mitochondria within cancer cells. Chem. Commun., 2019, 55, 6429-6432.