Publication Type:

Journal Article


Journal of Biomedical Materials Research - Part A, Volume 101, Number 10, p.2957-2966 (2013)



Acetic acid, adult, article, biocompatibility, Biocompatible Materials, blood clotting, blood clotting time, Blood Coagulation, blood compatibility, blood toxicity, chemistry, chitosan, Chitosan nanoparticles, Coagulation, Controlled drug delivery, cytokine, Cytokines, dielectric constant, Dispersing media, electrophoretic mobility, Erythrocytes, ethylenediaminetetraacetatic acid, Fourier Transform Infrared, gelation, glucose metabolism, Hemocompatibility, Hemolysis, Hemolytic activity, human, Humans, hydrodynamics, immune response, infrared spectroscopy, Intravenous applications, Lactic acid, macrogol, Materials Testing, nanoparticle, Nanoparticles, partial thromboplastin time, particle size, pH, Platelet aggregation, Platelets, polyvinyl alcohol, prothrombin time, Spectroscopy, static electricity, stereospecificity, surface charge, Surface chemistry, Surface modifiers, Surface properties, Thermogravimetry, thrombocyte adhesion, thrombocyte aggregation, thrombogenicity, unclassified drug, Young Adult, zeta potential


The increasing interest in using chitosan nanoparticles for controlled drug delivery is hampered by its blood incompatibility, especially for intravenous applications. This study investigated the effects of processing solvents (acetic acid/lactic acid), dispersing media (acidic medium/saline), and surface modifiers (polyethylene glycol, polyvinyl alcohol, and ethylenediaminetetraacetatic acid) on the hemocompatibility of chitosan. Blood compatibility of chitosan nanoparticles prepared by ionotropic gelation with altered surface chemistry was evaluated by assessing their hemolytic activity, platelet aggregation, coagulation, and cytokine induction. It was observed that nanoparticles prepared in lactic acid and dispersed in saline did not show hemolysis, platelet aggregation, or coagulation, whereas nanoparticles prepared in acetic acid showed strong hemolysis. Surface modifiers were not observed to significantly affect blood compatibility, with the exception of EDTA, which delayed blood clotting times. Thus, chitosan nanoparticles prepared in lactic acid and dispersed in saline may be an ideal nanocarrier for parenteral applications. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 101A:2957-2966, 2013. Copyright © 2013 Wiley Periodicals, Inc., a Wiley Company.


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Cite this Research Publication

, “Hematotoxicological analysis of surface-modified and -unmodified chitosan nanoparticles”, Journal of Biomedical Materials Research - Part A, vol. 101, pp. 2957-2966, 2013.