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February 2 – 3, 2026

Immune Symposia On Advanced Immune Cell Isolation, Characterization, And Sorting Using Bigfoot Flow Cytometer

In collaboration with ThermoFisher Scientific

Amrita Research Center Delhi NCR,
Amrita Vishwa Vidyapeetham

Proposed Theme Focus

This symposium will advance translational immunology by training clinical faculty, researchers and scholars in advanced flow
cytometry platforms, specifically the Bigfoot Flow Cytometer, with emphasis on immune cell isolation, phenotypic characterization, and cell sorting for diagnostic, therapeutic, and vaccine development applications. The program aligns with ARCD vision of bridging fundamental discoveries with real-world clinical solutions by equipping participants with practical skills in state-of-the-art immunophenotyping and immune sorting technologies.

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Objectives

  • To build expertise in advanced flow cytometry for immune cell characterization & isolation
  • To develop research competency in multi-parametric immune profiling
  • To establish pathways for regulatory compliance and clinical readines

Hands-on Sessions Using Lab Infrastructure

  • Flow Cytometry: Cell phenotyping, immune profiling, and quality control
  • Cell Sorting: Isolation of specific immune populations for downstream applications
  • Data Analysis: Software-driven interpretation of multi-parametric datasets
  • Protocol Development: Standardized pipelines for translational research

Registration Fee

Registration Fee for Students

₹1,000 + 18% GST

Registration Fee for Faculty

₹2,000 + 18% GST

Last Date of Registration

20th Jan 2026

Send your application along with payment confirmation details to the Workshop Secretary and Administrative Manager.
ashishk.vyas@fbd.amrita.edu/ researchadmin@fbd.amrita.edu

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Patrons

Prof. Shantikumar V Nair
Associate Provost, Head and Dean
Amrita Research Center, Delhi NCR
Amrita Vishwa Vidyapeetham, Faridabad Campus

Prof. Amit Kumar Dinda
Research Director
Amrita Research Center, Delhi NCR
Amrita Vishwa Vidyapeetham, Faridabad Campus

committee

Dr. Ashish Kumar Vyas 

Organization Secretary

Organizing Team

Dr. Anubhav Pandey, Dr. Pankaj Jha, Dr. Sreeja Roy, Dr. Bhargab Kalita, Dr. Aparup Patra, Dr. Anuj Dilwaria, Dr. Ansar Fasaludeen and Mr. Vikas Jha

Day 1: Foundations & Practical Applications In Immune Cell Isolation

Time Programme
08:30 AM – 09:30 AM Registration
Session 1 – Inauguration
09:30 AM – 09:35 AM Pooja Ceremony
09:35 AM – 09:50 AM Welcome Address and Introduction of Participants
09:50 AM – 10:00 AM Overview of Symposium Objectives
10:00 AM – 10:30 AM Keynote: From Immune Discovery to Diagnostic Innovation – Role of Advanced Flow Cytometry
10:30 AM – 10:45 AM Tea Break
Session 2 – Flow Cytometry in Translational Immunology
10:45 AM – 11:15 AM Immunophenotyping with Bigfoot Flow Cytometer: Multi-Parameter Panel Design
11:15 AM – 11:45 AM Advanced Immune Cell Isolation Techniques: Principles and Best Practices
11:45 AM – 12:15 PM Flow Cytometry in Clinical Diagnostics: Disease Monitoring and Biomarker Discovery
12:15 PM – 01:00 PM Panel Discussion
01:00 PM – 02:00 PM Lunch
Lab Session 1 (Groups A & B Rotate)
02:00 PM – 03:30 PM

Lab 1A (Group A): Introduction to Bigfoot Flow Cytometer – Instrumentation, Compensation & Panel Design


Lab 1B (Group B): Immune Cell Isolation Techniques – Magnetic Bead Separation & Density Gradient Protocols
03:30 PM – 03:45 PM Tea Break
03:45 PM – 05:15 PM Hands-on Rotation (Switch A & B)
Day 1 – Hands-on Sessions  
Lab 1A:  Introduction to Bigfoot Flow
Cytometer – Instrumentation,
Compensation & Panel Design		 Lab 1B: Immune Cell Isolation Techniques
  • Instrument orientation and software navigation
  • Voltage optimization and compensation strategies
  • Antibody panel design for multi-parametric analysis
  • Quality control (QC) checks and standardization protocols
  • Magnetic bead-based isolation (negative & positive selection)
  • Density gradient centrifugation for peripheral blood mononuclear cell (PBMC) isolation
  • Purity assessment and viability testing
  • Protocol troubleshooting and optimization

Day 2: Translational Readiness, Immune Cell Sorting & Advanced Applications

Time Programme
Session 3- Advanced Analysis & Clinical Translation  
09:30 AM – 10:00 AM Immune Profiling in Vaccine Research: Monitoring Immune Responses
10:00 AM – 10:30 AM Quality Control, Standardization, and Regulatory Pathways in Flow Cytometry
10:30 AM – 10:45 AM Panel Discussion
10:45 AM – 11:00 AM Tea Break
11:00 AM – 11:30 AM Investment Scenarios and Grant Opportunities for Translational Immunology
11:30 AM – 12:00 PM Panel Discussion: Academia–Industry Collaborations for Immune Cell Technology Deployment
12:00 PM – 01:00 PM Live Training on Flow Data Analysis using FlowJO Software
01:00 PM – 02:00 PM Lunch
Lab Session 2 (Groups A & B Rotate)  
02:00 PM – 03:15 PM

Lab 2A (Group A): Advanced Flow Cytometry – Multi-Parametric Immune Subset Profiling & Data Analysis

Lab 2B (Group B): Fluorescence-Activated Cell Sorting (FACS) – Isolation of Specific Immune Populations
03:15 PM – 03:30 PM Tea Break
03:30 PM – 04:45 PM Hands-on Rotation (Switch A & B)
04:45 PM – 05:30 PM Closing Ceremony – Feedback, Group Photo, Closing Remarks & Certificate Distribution
Day 2 – Hands-on Sessions  
Participants will engage in structured, small-group practical sessions designed for experiential learning and competency building:  
Lab 2A: Advanced Flow Cytometry – Multi-Parametric Immune Subset Profiling & Data Analysis Lab 2B: Fluorescence-Activated Cell Sorting (FACS) – Isolation of Specific Immune Populations
  • Gating strategies for immune cell identification (T cells, B cells, and NK cells)
  • Fluorescent compensation and spillover correction.
  • Bigfoot software tools for data visualization and statistical analysis Analysis of disease-specific immune phenotypes
  • Cell sorting principles and instrument setup
  • Purity and recovery optimization
  • Downstream applications (RNA extraction, functional assays, culture)
  • Safety protocols and ethical considerations
Participant Division:

Participants will be divided into two groups (A & B) and will rotate between lab stations on both days to ensure equal exposure and comprehensive learning

Target Participants:
  • Early-career researchers, clinicians, and PhD scholars in biomedical sciences.
  • Translational research teams from academia and industry.
Expected Outcomes:
  • Basic Understanding of Immune Cell Profiling and Sorting
  • Translational Research Competency in Immunology
  • Hands-On Technical Proficiency and Analytical Skills
Support requirements:
  • Logistical support for travel and accommodation of invited faculty
  • Funding for workshop materials, antibodies, and reagents 
  • Technical support from ThermoFisher Scientific for instrument and software assistance
  • Promotion through academic networks and institutional platforms
  • Administrative support for participant registration and documentation
  
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