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Course Detail

Course Name Immunology & Microbiology Lab
Course Code 25NBT583
Program M. Sc. Nanobiotechnology (NBT)
Semester 2
Credits 2
Campus Kochi

Syllabus

Lab Contents:

Unit 1 (Lab sessions = 2)

Bacterial and fungal culture medium preparation, Pure culture technique (eg: streaking and sub culturing), Gram staining, Fungal staining, Motility assay

Unit 2 (Lab sessions = 3)

Bacterial growth curve by measuring turbidity and viable count

Unit 3 (Lab sessions = 5)

Antibiotic sensitivity assay; MIC and MBC determination, Biofilm assay, MBIC and MBEC determination, Phage titration assay

Unit 4 (Lab sessions = 7)

Observation & Identification of Immune Cells, Observation and identification of the structure of lymphoid organs, Blood grouping, Separation of Mononuclear cells using density gradient centrifugation, Isolation of Monocytes from the Mononuclear Cells, Induction of Monocytes to M1 and M2 Macrophages, Evaluation of inflammatory and anti-inflammatory cytokines produced by M1 and M2 macrophages through ELISA.

Objectives and Outcomes

Pre-requisites: Basic understanding of experimental research

Total number of lab sessions: 15

COURSE OUTCOME:

  • Provide the students the knowledge about practical skills on basic microbiology.
  • Students will learn about different antimicrobial activity assays.
  • Students will develop an understanding about components of immune system and their function.
  • The students will learn about different antigen and antibody interaction assays.

TEXT BOOKS:

  1. Practical Handbook of Microbiology-Lorrence H Green, Emanuel Goldman by CRC Press, 4th Edition, ISBN 9780367567637.
  2. Practical Immunology-Frank C. Hay, Olwyn M.R. Westwood, Fourth Edition, Blackwell Science.

COURSE OUTCOME:

CO1: Provide the students the knowledge about practical skills on basic microbiology.

CO2: Students will learn about different antimicrobial activity assays.

CO3: Students will develop an understanding about components of immune system and their function.

CO4: The students will learn about different antigen and antibody interaction assays.

Program Outcomes (POs)

PO1: Bioscience Knowledge

PO2: Problem Analysis

PO3: Design/Development of Solutions

PO4: Conduct Investigations of complex problems

PO5: Modern tools usage

PO6: Bioscientist and Society

PO7: Environment and Sustainability

PO8: Ethics

PO9: Individual & Team work

PO10: Communication

PO11: Project management & Finance

PO12: Lifelong learning

CO-PO Mapping Table:

COs

PO1

PO2

PO3

PO4

PO5

PO6

PO7

PO8

PO9

PO10

PO11

PO12

CO1

3

2

–

2

2

2

–

–

–

–

–

2

CO2

3

3

2

2

3

2

–

–

–

–

–

2

CO3

3

2

–

–

–

3

–

–

–

–

–

2

CO4

3

2

2

2

3

–

–

–

–

–

–

2

PROGRAM SPECIFIC OUTCOMES (PSO):

PSO1: Mastery of Nanomaterial Science and Characterization

PSO2: Understanding of Cellular and Molecular Systems

PSO3: Application of Nanobiotech in Diagnostics and Therapy

PSO4: Experimental Design and Data Analysis

PSO5: Research Competency and Methodological Rigor

PSO6: Industry and Career Readiness

CO–PSO Mapping Table:

COs

PSO1

PSO2

PSO3

PSO4

PSO5

PSO6

CO1

–

3

–

2

2

2

CO2

–

2

3

2

3

3

CO3

–

3

2

–

2

2

CO4

–

3

3

2

2

2

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