Course

Unit 1

Polymeric Nanoparticles: Synthesis of alginate nano and micro particles; characterization of particle size by Dynamic Light Scattering (DLS) and Zeta analysis

Synthesis of chitosan nanoparticles; characterization of particle size by Dynamic Light Scattering (DLS) and Zeta analysis

Unit 2

Synthesis of ceramic Silica Nanoparticles and characterization using TEM. Structural characterization of crystalline and amorphous nanomaterials using X-ray diffraction spectrometer (XRD), Thermal characterization of polymers using Thermogravimetric – Differential thermal Analysis (TGA-DTA)

Unit 3

Electrospinning: Fabrication of electrospun PVA nanofibres and microfibers; characterization of fibers morphology and diameter using SEM. Characterization of polymeric and inorganic samples (chitosan and TiO₂ using Raman Spectroscopy Using Raman spectroscopy)

Pre-requisites: Basic understanding of experimental research

Total number of lab sessions: 15

COURSE OUTCOME:

After successful completion of the course, students will be able to:

• Understand the principles of Dynamic Light Scattering technique in estimating the particle size and zeta potential. How zeta potential is related to the particle stability
  • Understand the synthesis of polymeric Alginate nanoparticles and the role of reaction parameters that varies the particle size
  • Understand the synthesis of Chitosan Nanoparticles and characterization using DLS and Zeta Potential.
• Understand the basics of vibrational spectroscopy FTIR and characterize alginate and chitosan.
• Understand the synthesis of ceramic Silica Nanoparticles and characterization using TEM.
• Understand the basics of XRD in characterizing crystalline and amorphous samples.
• Understand the thermal and mechanical characterization of polymeric samples CaCO₃ and chitosan.
• Understand the electrospinning technique and parameters that influence the formation of micro and nano fibers.
• Understand the basics of Raman spectroscopy in characterizing in polymers and inorganics samples (chitosan and TiO₂).

COURSE OUTCOMES:

CO1 Synthesis of Polymeric nano and micro particles; characterization of particle size by Dynamic Light Scattering (DLS) and Zeta analysis

CO2 Fabrication of electrospun PVA nanofibres and microfibers; characterization of fibers morphology and diameter using SEM

CO3 Structural characterization of crystalline and amorphous nanomaterials using X-ray diffraction spectrometer (XRD), Characterization of polymeric and inorganic samples using Raman Spectroscopy Using Raman spectroscopy

CO4 Thermal characterization of polymers using Thermogravimetric – Differential thermal Analysis (TGA-DTA), Characterization of materials properties by studying stress-strain curve using Rheometer.

PROGRAM SPECIFIC OUTCOMES:

PO1:  Engineering/Bioscience Knowledge

PO2: Problem Analysis

PO3: Design/Development of Solutions

PO4: Conduct Investigations of complex problems

PO5: Modern tools usage

PO6: Bioscientist and Society

PO7: Environment and Sustainability

PO8: Ethics

PO9: Individual & Team work

PO10: Communication

PO11: Project management & Finance

PO12: Lifelong learning

CO-PO MAPPING:

Program Specific Outcomes (PSO)

PSO1: Mastery of Nanomaterial Science and Characterization

PSO2: Understanding of Cellular and Molecular Systems

PSO3: Application of Nanobiotech in Diagnostics and Therapy

PSO4: Experimental Design and Data Analysis

PSO5: Research Competency and Methodological Rigor

PSO6: Industry and Career Readiness

CO–PSO Mapping Table:

 References:

• Polymer Nanoparticles for Nanomedicines: A Guide for their Design, Preparation and Development; Christine Vauthier, Springer
• Nano: The Essentials; T. Pradeep, Tata McGraw-Hill.
• Nanostructures & Nanomaterials: Synthesis, Properties & Applications; Guozhong Cao, Imperial College Press.