Back close
Admissions

Course Detail

Course Name Laboratory Techniques in Stem Cell Research Lab
Course Code 25SCR581
Program M. Sc. Stem Cell Technology and Regenerative Medicine
Semester 1
Credits 2
Campus Kochi

Syllabus

Unit 1

(Lab sessions = 5)

Isolation of Mesenchymal Stem Cells (MSCs) from adipose tissue, Cultivation and morphological observation of adherent MSCs, Assessment of growth kinetics and morphology tracking.

Unit 2

(Lab sessions = 3)

Isolation of Hematopoietic Stem Cells (HSCs) using Ficoll-based density gradient from cord blood, Demonstration of CD34⁺ cell enrichment using Magnetic-Activated Cell Sorting (MACS).

Unit 3

(Lab sessions = 8)

Flow cytometry for analysis of positive and negative surface markers in MSCs and HSCs,
Tri-lineage differentiation of MSCs: Adipogenic, osteogenic, and chondrogenic induction (experimental setup and analysis), Histological staining for lineage-specific markers: Oil Red O for adipogenesis, Alizarin Red for osteogenesis, Alcian Blue for chondrogenesis, Interpretation of flow cytometry data and staining results, Preparation of experimental reports and discussion of observations

Introduction

Pre-requisites: Basic practical understanding in biology
Total number of hours: 30

Preample : This practical course introduces students to key laboratory techniques in stem cell research, focusing on the isolation, characterization, and differentiation of Mesenchymal and Hematopoietic Stem Cells. Through hands-on modules, students will learn methods such as density gradient separation, magnetic sorting, flow cytometry, and trilineage differentiation assays, enabling them to gain foundational skills essential for research in regenerative medicine and cell-based therapies.

Objectives and Outcome

Course Outcome

CO1: Explain the fundamental principles of stem cell isolation and characterization from various biological sources.

CO2: Demonstrate proficiency in isolating mesenchymal stem cells (MSCs) from adipose tissue and hematopoietic stem cells (HSCs) using Ficoll-based density gradient methods.

CO3: Perform phenotypic characterization of MSCs and HSCs using flow cytometry and magnetic cell sorting (MACS).

CO4: Set up and analyze tri-lineage differentiation assays (adipogenic, osteogenic, chondrogenic) including histological staining techniques.

CO5: Track growth kinetics, analyze morphological features, and interpret experimental data to evaluate stem cell behavior and identity.

Program Outcomes (PO)

PO1: Bioscience Knowledge

PO2: Problem Analysis

PO3: Design/Development of Solutions

PO4: Conduct Investigations of complex problems

PO5: Modern tools usage

PO6: Bioscientist and Society

PO7: Environment and Sustainability

PO8: Ethics

PO9: Individual & Team work

PO10: Communication

PO11: Project management & Finance

PO12: Lifelong learning

PO1

PO2

PO3

PO4

PO5

PO6

PO7

PO8

PO9

PO10

PO11

PO12

CO1

3

3

–

–

3

3

3

–

1

1

–

2

CO2

3

3

1

1

3

3

3

2

1

1

–

2

CO3

1

1

1

2

3

2

3

1

2

1

–

2

CO4

1

1

1

1

1

3

3

2

3

2

–

2

CO5

2

2

1

1

2

3

3

3

3

2

–

2

Program Specific Outcomes (PSO)

PSO1. Demonstrate comprehensive knowledge of stem cell biology and their clinical and research relevance.

PSO2. Apply core laboratory techniques for stem cell isolation, characterization, and manipulation.

PSO3. Integrate principles of tissue engineering and biomaterials for regenerative applications.

PSO4. Analyze drug delivery, pharmacokinetics, and bioinformatics relevant to stem cell-based therapies.

PSO5. Evaluate ethical, regulatory, and translational aspects of stem cell product development.

PSO6. Bridge basic science with translational approaches in regenerative medicine and gene therapy.

PSO7. Design and interpret experimental strategies for stem cell-based disease

modeling and preclinical studies.

C

PSO1

PSO2

PSO3

PSO4

PSO5

PSO6

PSO7

C O

CO 1

1

2

1

–

–

1

–

CO 2

2

3

1

–

–

1

–

CO 3

3

3

1

–

–

1

–

CO 4

3

3

1

–

–

2

–

CO 5

3

3

1

–

–

2

2

Evaluation Pattern

Evaluation Pattern: 30 + 70 = 100

Internal Assessment – 30% 

Records 

Evaluation 

30% 

30%

End Semester Examination- 50% 

Practical

Exam 

60% 

Assessment

Viva 

10%

70%

Text Book/ Reference

Text Book/ Reference

  • Culture of Animal Cells, A manual of basic technique and specialized applications, Sixth Edition R. Ian Freshney

DISCLAIMER: The appearance of external links on this web site does not constitute endorsement by the School of Biotechnology/Amrita Vishwa Vidyapeetham or the information, products or services contained therein. For other than authorized activities, the Amrita Vishwa Vidyapeetham does not exercise any editorial control over the information you may find at these locations. These links are provided consistent with the stated purpose of this web site.

Admissions Apply Now