| Course Name | Analytical Techniques and Imaging in Stem Cell Research-Lab |
| Course Code | 25SCR583 |
| Program | M. Sc. Stem Cell Technology and Regenerative Medicine |
| Semester | 2 |
| Credits | 2 |
| Campus | Kochi |
Analytical techniques in stem cell research: Flow cytometry basics, fluorophore selection and compensation, data acquisition and gating strategies, RNA isolation, cDNA synthesis, qPCR for gene expression analysis, interpretation of gene expression data, in vitro differentiation assays, marker analysis using flow cytometry and qPCR.
Imaging techniques in stem cell research: Principles of immunostaining, surface and intracellular marker detection, antibody selection and optimization, fluorescence microscopy, electron Microscopy, confocal microscopy, live-cell imaging basics, interpretation of imaging data.
Pre-requisites: Basic practical understanding in biology
Total number of hours: 30
Preamble
This course provides hands-on exposure to essential analytical and imaging techniques employed in stem cell research. Emphasizing practical proficiency, the course introduces students to methods for cell characterization, gene expression analysis, and visualization of stem cell behavior. Through modules on flow cytometry, qPCR, immunostaining, and advanced microscopy, students will gain the technical foundation necessary for experimental design and data interpretation in both basic and translational stem cell research.
Course Outcomes (COs)
CO1. Use flow cytometry for stem cell phenotyping and differentiation analysis.
CO2. Perform qPCR for gene expression profiling in stem cell studies.
CO3. Apply immunostaining to detect key stem cell markers.
CO4. Operate fluorescence and confocal microscopes for stem cell imaging.
CO5. Interpret data from analytical and imaging techniques in stem cell research.
Program Outcomes (PO)
PO1: Bioscience Knowledge
PO2: Problem Analysis
PO3: Design/Development of Solutions
PO4: Conduct Investigations of complex problems
PO5: Modern tools usage
PO6: Bioscientist and Society
PO7: Environment and Sustainability
PO8: Ethics
PO9: Individual & Team work
PO10: Communication
PO11: Project management & Finance
PO12: Lifelong learning
0 – No affinity; 1 – low affinity; 2 – Medium affinity; 3 – High affinity
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C |
PO1 |
PO2 |
PO3 |
PO4 |
PO5 |
PO6 |
PO7 |
PO8 |
PO9 |
PO10 |
PO11 |
PO12 |
|
C O |
||||||||||||
|
CO 1 |
3 |
2 |
2 |
3 |
3 |
1 |
0 |
1 |
1 |
1 |
1 |
2 |
|
CO 2 |
3 |
2 |
2 |
3 |
3 |
1 |
0 |
1 |
1 |
1 |
1 |
2 |
|
CO 3 |
3 |
2 |
2 |
3 |
3 |
1 |
0 |
1 |
1 |
1 |
1 |
2 |
|
CO 4 |
3 |
2 |
2 |
3 |
3 |
1 |
0 |
1 |
1 |
1 |
1 |
2 |
|
CO 5 |
3 |
3 |
2 |
3 |
3 |
1 |
0 |
1 |
1 |
2 |
1 |
3 |
Program Specific Outcomes. (PSO)
PSO1. Demonstrate comprehensive knowledge of stem cell biology and their clinical and research relevance.
PSO2. Apply core laboratory techniques for stem cell isolation, characterization, and manipulation.
PSO3. Integrate principles of tissue engineering and biomaterials for regenerative applications.
PSO4. Analyze drug delivery, pharmacokinetics, and bioinformatics relevant to stem cell-based therapies.
PSO5. Evaluate ethical, regulatory, and translational aspects of stem cell product development.
PSO6. Bridge basic science with translational approaches in regenerative medicine and gene therapy.
PSO7. Design and interpret experimental strategies for stem cell-based disease
modeling and preclinical studies.
|
– |
PSO1 |
PSO2 |
PSO3 |
PSO4 |
PSO5 |
PSO6 |
PSO7 |
|
C O |
|||||||
|
CO 1 |
3 |
3 |
– |
– |
– |
2 |
– |
|
CO 2 |
3 |
3 |
– |
– |
– |
2 |
– |
|
CO 3 |
3 |
3 |
– |
– |
– |
2 |
– |
|
CO 4 |
3 |
3 |
– |
– |
– |
2 |
– |
|
CO 5 |
3 |
3 |
– |
– |
– |
2 |
– |
Evaluation Pattern: 30 + 70 = 100
| Internal Assessment – 30% | ||
| Records | Evaluation | 30% |
| 30% | ||
| End Semester Examination- 50% | ||
| Practical | Exam | 60% |
| Assessment | Viva | 10% |
| 70% | ||
Textbooks / References
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