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Course Detail

Course Name Recombinant DNA Technology
Course Code 25SCR543
Program M. Sc. Stem Cell Technology and Regenerative Medicine
Semester 2
Credits 3
Campus Kochi

Syllabus

Unit1

(5 Lectures)

Restriction enzymes and cloning vectors: Host controlled restriction modification,
Restriction endonucleases – types and classification, modifying enzymes used in molecular cloning – methylases, polymerases, ligases, kinases, phosphatases and nucleases

Unit 2

(10 Lectures)

Vectors and Hosts: Vectors for cloning, expression vectors (plasmids, lambda phage vectors, cosmids, BAC & YAC); Host organisms used in r-DNA technology: E. coli, Yeast, Insect cells as model organisms.

Unit 3

(10 Lectures)

Cloning, Construction of Genomic and cDNA libraries: Introduction to cloning
(conventional & recombination based: traditional cloning, TA cloning, Gateway cloning etc), vGene transfer techniques: Transformation, transfection and transduction, Strategies for construction of genomic libraries, Strategies for construction of cDNA libraries.

Unit 4

(10 Lectures)

Selection and characterization of recombinant clones: Genetic Selection- insertional
inactivation and alpha complementation, Labeling of nucleic acids, Immunological probes, Selection of recombinant clones-hybridization techniques, colony hybridization & library screening, hybrid arrest & hybrid release translation, DNA sequencing methods, DNA arrays.

Unit 5

(10 Lectures)

Advanced techniques and applications: Genome editing- CRISPR-Cas system, TALENs &amp ZFNs, Site directed mutagenesis and RNA interference, Recombinant vaccines, Recombinant antibodies (Fab, scFv, sdAb), Disease diagnosis, Gene therapy- technologies, applications and regulations Next generation sequencing- principle, types and applications.

Introduction

Pre-requisites: Undergraduate level basic biology, biotechnology

Total number of classes: 30

Objectives and Outcomes

Course Outcome

CO1 Understand the fundamentals of molecular cloning

CO2 Understand the technical know-how on versatile techniques in recombinant DNA technology.

CO3 Gain knowledge of tools and strategies used in molecular cloning

CO4 Understand how to select and characterize recombinant clones

CO5 Understand basic and advanced molecular cloning concepts, applications and techniques

Programme Outcomes (PO)

(As given by NBA and ABET)

PO1: Bioscience Knowledge

PO2: Problem Analysis

PO3: Design/Development of Solutions

PO4: Conduct Investigations of complex problems

PO5: Modern tools usage

PO6: Bioscientist and Society

PO7: Environment and Sustainability

PO8: Ethics

PO9: Individual & Team work

PO10: Communication

PO11: Project management & Finance

PO12: Lifelong learning

c

PO1

PO2

PO3

PO4

PO5

PO6

PO7

PO8

PO9

PO10

PO11

PO12

C O

CO 1

3

–

2

–

2

–

–

2

–

–

–

2

CO 2

3

1

2

2

3

–

–

–

–

–

–

1

CO 3

3

2

1

1

3

2

–

–

–

–

–

1

CO 4

3

2

3

3

–

1

–

–

–

–

–

1

CO 5

3

–

–

2

3

1

–

–

–

–

–

1

Program Specific Outcomes (PSO)

PSO1. Demonstrate comprehensive knowledge of stem cell biology and their clinical and research relevance.

PSO2. Apply core laboratory techniques for stem cell isolation, characterization, and manipulation.

PSO3. Integrate principles of tissue engineering and biomaterials for regenerative applications.

PSO4. Analyze drug delivery, pharmacokinetics, and bioinformatics relevant to stem cell-based therapies.

PSO5. Evaluate ethical, regulatory, and translational aspects of stem cell product development.

PSO6. Bridge basic science with translational approaches in regenerative medicine and gene therapy.

PSO7. Design and interpret experimental strategies for stem cell-based disease

modeling and preclinical studies.

 

PSO1

PSO2

PSO3

PSO4

PSO5

PSO6

PSO7

C O

CO 1

–

–

–

–

–

2

–

CO 2

–

–

–

–

–

3

–

CO 3

–

–

–

–

–

2

–

CO 4

–

–

–

–

–

2

–

CO 5

–

–

–

–

–

–

–

 

Evaluation Pattern

Evaluation Pattern: 50+50 = 100

Internal Assessment – 50% 
Periodical 1  Exam  20% 
Periodical 2  Exam  20% 
Continuous Assessment Assignment/ Test/ Quiz 10%
    50%
End Semester Examination- 50%
Theory Exam   50%
    50%
Total 100%

Textbooks/ References

Textbooks:

  1. Principles of Gene Manipulation, Sandy B. Primrose, Richard Twyman, Bob Old, Wiley, 08-Feb-2002
  2. From Genes to Genomes: Concepts and Applications of DNA Technology. Jeremy W Dale and Malcom von Schantz 2002 John Wiley & Sons, Ltd.

Reference

  1. Gene Therapy Technologies, Applications and Regulations: From Laboratory to Clini. Anthony Meager 1999 John Wiley & Sons, Ltd.

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