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Course Detail

Course Name Molecular Biology
Course Code 25SCR532
Program M. Sc. Stem Cell Technology and Regenerative Medicine
Semester 1
Credits 3
Campus Kochi

Syllabus

Unit 1

(Lectures 10)

DNA: Structure and function, Chromosome and chromatin, Genetic code, wobble hypothesis, RNA and types of RNA (rasiRNA, tasiRNA, nat-siRNA, piRNA), Proteins and their structure

Unit 2

(Lectures 10)

DNA replication and its regulation, Homologous and site-specific recombination, DNA repair

Unit 3

(Lectures 10)

Transcription and its regulation, Translation and its regulation, Gene structure, Repeats and clusters, Gene expression regulations: operon, Epigenetics

Unit 4

(Lectures 5)

Types of mutations, Genetic system of mitochondria

Unit 5

(Lectures 10)

Gene identification, promoter identification, Molecular biology techniques: Isolation and
Quantification of DNA/RNA, PCR, Reverse transcriptase PCR, Real Time PCR, DNA Sequence analysis, hybridization (southern, northern and western) and Sanger sequencing.

Introduction

Pre-requisites: Undergraduate level basic DNA biology

Total number of classes: 45

Preamble

This course will provide a thorough understanding about biology of DNA and how its regulations are brought about, its functional significance and how it is very much involved in the biochemistry and physiology of the cell.

Objectives and Outcomes

Course Outcome

CO1 Understand the structure and function of DNA, RNA and proteins

CO2 Understand the basics of DNA and RNA replication, transcription, translation

and DNA-repair systems

CO3 Understand how genetic switches work, the basics of gene regulation in prokaryotes and eukaryotes

CO4 Understand the consequences of different types of mutations and recombinations

CO5 Understand basic and advanced molecular biology concepts and techniques

PO1: Bioscience Knowledge

PO2: Problem Analysis

PO3: Design/Development of Solutions

PO4: Conduct Investigations of complex problems

PO5: Modern tools usage

PO6: Bioscientist and Society

PO7: Environment and Sustainability

PO8: Ethics

PO9: Individual & Team work

PO10: Communication

PO11: Project management & Finance

PO12: Lifelong learning

C

PO1

PO2

PO3

PO4

PO5

PO6

PO7

PO8

PO9

PO10

PO11

PO12

C O

CO 1

3

1

–

–

–

–

–

–

–

–

–

2

CO 2

3

1

–

–

1

–

–

–

–

–

–

1

CO 3

3

2

1

2

2

1

–

–

–

–

–

1

CO 4

3

3

1

2

2

–

–

–

–

–

–

2

CO 5

3

1

2

1

3

2

–

–

–

–

–

1

Program Specific Outcomes. (PSO)

PSO1. Demonstrate comprehensive knowledge of stem cell biology and their clinical and research relevance.

PSO2. Apply core laboratory techniques for stem cell isolation, characterization, and manipulation.

PSO3. Integrate principles of tissue engineering and biomaterials for regenerative applications.

PSO4. Analyze drug delivery, pharmacokinetics, and bioinformatics relevant to stem cell-based therapies.

PSO5. Evaluate ethical, regulatory, and translational aspects of stem cell product development.

PSO6. Bridge basic science with translational approaches in regenerative medicine and gene therapy.

PSO7. Design and interpret experimental strategies for stem cell-based disease

modeling and preclinical studies.

C

PSO1

PSO2

PSO3

PSO4

PSO5

PSO6

PSO7

C O

CO 1

–

–

–

–

–

2

–

CO 2

–

–

–

–

–

2

–

CO 3

–

–

–

–

–

3

–

CO 4

–

2

–

–

–

3

–

CO 5

–

3

–

–

–

3

–

Evaluation Pattern

Evaluation Pattern: 50+50 = 100

Internal Assessment – 50% 

Periodical 1 

Exam 

20% 

Periodical 2 

Exam 

20% 

Continuous Assessment 

Assignment/Test/Quiz

10% 

50%

End Semester Examination- 50% 

Theory Exam 

50% 

50%

Total

100%

Textbook/ References

Textbook:

Lewin’s Essential Genes, Jocelyn E. Krebs, Benjamin Lewin, Elliott S. Goldstein, Stephen T. Kilpatrick, Jones& Bartlett Publishers, 2013 – Science – 847 pages

References

Molecular Biology of the Gene, Seventh Edition, James D. Watson, Cold Spring Harbor Laboratory; Tania A. Baker, Massachusetts Institute of Technology; Alexander Gann, Cold Spring Harbor Laboratory; Michael Levine, University of California, Berkeley; Richard Losick, Harvard University,2013

 

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