Course

• Preparation of Laboratory Solutions and Buffers.
• Verification of Beer-lamberts Law using Potassium Dichromate.
• Estimation of Amino acids by Ninhydrin Method.
• Separation of Amino acids using TLC.
• Isoelectric Precipitation of Casein from Milk.
• Qualitative Analysis of Carbohydrates.
• Qualitative Analysis of Amino acids.
• Estimation of Reducing Sugar using DNS Method.

LEARNING OBJECTIVES:

This course deals with basic biochemical calculations and preparations of various reagents, qualitative and quantitative analysis of both carbohydrates and amino acids, and chromatography techniques.

COURSE OUTCOMES:

After completing the course, students shall be able to 

CO1. Students will get practical exposure to common buffer and reagent preparations

CO2. Skill development for students on handling basic laboratory biochemical equipment’s (pH meter, colorimeter, centrifuge, micropipettes).

CO3. Developing qualitative and quantitative analytical skills on biomolecules.

REFERENCES: 

1. “Experimental Biochemistry”, Beedu Sashidhar rao, Vijay Deshpande, I K International Pvt.    Ltd., ISBN 81-88237-41-8 1^st edition 2005.
2. Laboratory Manual in Biochemistry; J.Jayaraman, New Age International Private Limited, Second edition 2011.