Course

Unit 1

Genomics: Basics of genome sequence and annotation, Functional Genomics.

Unit 2

Transcriptomics: Definition, Analytical techniques, PCR and QRT PCR, Microarrays, snRNA, snoRNA, tRNA, rRNA and miRNA, RNA Sequencing, RNA Splicing, Post- transcriptional regulation of gene expression.

Unit 3

Proteomics: Introduction to proteomics, Importance of mass spectrometry in Proteomics, Basic concepts and Instrumentations, Electrospray (ESI), Matrix Assisted Laser Desorption and Ionization (MALDI), Quadrupole, Ion trap, Time-of Flight, Fourier transform ion cyclotron resonance (FT-ICR), Orbitrap, Electron multiplier horn, Microchannel plate.

Unit 4

Protein separation and detection techniques: Electrophoresis, High-performance liquid chromatography (HPLC), Peptide mass finger printing, Tandem mass spectrometry, Major Protein identification softwares-Mascot and Sequest.

Unit 5

Metabolomics: Introduction to metabolomics, Workflows of metabolome analysis.

LEARNING OBJECTIVES:

The major aim of this undergraduate course is to provide basic theoretical knowledge in the field of Genomics, Transcriptomics, Proteomics and Metabolomics.

COURSE OUTCOMES:

After completing the course, students shall be able to

CO1. Discriminate the differences of different genome.
CO2. Explain the different concepts and mechanisms involved in genomics and transcriptomics.

CO3. Describe methodologies and techniques associated with omics.
CO4. Describe basics of Mass Spectrometry, Proteomics and Metabolomics.
CO5. Recall major separation techniques in Proteomics and Metabolomics.
CO6. Compare and discuss workflows and strategies for protein identification and characterization.

REFERENCES:1. Genomes, Terence A. Brown, Garland Publishing, 3rd Ed., 2007.2. Molecular Biology, Robert F. Weaver, McGraw-Hill, 5th Ed., 2012.3. Molecular Biology of the Gene, James D. Watson, Tania A. Baker, Stephen P. Bell, Pearson, 5th Ed., 2006.4. Introduction to Proteomics: Tools for the New Biology, Daniel C. Liebler, Humana Press, 1st Ed., 2001.5. Mass Spectrometry for Biotechnology, Gary Siuzdak, Academic Press, 1st Ed., 1996.6. Principles and Techniques of Biochemistry and Molecular Biology, Keith Wilson and John Walker, Cambridge University Press, 7th Ed., 2010.7. Mass spectrometry-based metabolomics: A practical Guide, Sastia P. Putri and Eiichiro Fukusaki, CRC Press, 1st Ed., 2015.