Course

Unit I

EnzymesEnzyme nomenclature, Enzyme commission numbers, Enzymes in bioanalytical chemistry, Enzyme kinetics – Enzyme activators, Enzyme inhibitors.

Unit II

Quantification of Enzymes and their SubstratesInstrumental methods, optical detection – absorbance, Fluorescence, Luminescence, Nephlometry Electrochemical detection – amperometry, potentiometry, conductometry, other detection methods – radiochemical, manometry, calorimetry.

Unit III

Immobilized EnzymesImmobilization methods – nanopolymerizing covalent immobilization, Crosslinking with bifunctional reagents. Properties of immobilized enzymes, immobilized enzyme reactions, theoretical treatment of packed bed enzyme reactors.

Unit IV

AntibodiesStructural and functional properties of antibodies, polyclonal and monoclonal antibodies. Antibody-antigen interactions, analytical application of secondary antibody-antigen.

Unit V

BiosensorsResponse of enzyme-based biosensors, examples of Biosensor Configuration, Ferrocene-mediated amperometric glucose sensor, potentiometric biosensor for phenyl acetate, potentiometric immunosensor for digoxin, optical biosensor for glucose-based on fluorescence energy transfer, piezoelectric sensor for nucleic acid detection, enzyme thermistors.

TEXTBOOKS1.Susan R. Mikkelsen, and Eduardo Corton Bioanalytical Chemistry, 1st edition, Wiley Interscience, 2003.2.Andres Manz, Nicole Pamme and Dimitri Lossifidis, Bioanalytical Chemistry, World Scientific Publishing Company, 2004.REFERENCE1. Robert W. Cattrall, Chemical Sensors, Oxford University Press, 1997.