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Course Detail

Course Name Host-pathogen Interaction and Human Microbiome
Course Code 25BIO432
Program B.Sc. (Hons.) Microbiology
Semester 7
Credits 3
Campus Amritapuri

Syllabus

Unit 1: Host-Pathogen Interactions: Immune Evasion, Intracellular Survival, and Signaling

Pathogenic microbes and their interactions with the host immune system. Molecular and cellular strategies pathogens use to evade host defenses, including antigenic variation, inhibition of phagocytosis, complement evasion, and manipulation of cytokine responses, epigenetic modifications. How Mycobacterium tuberculosis, Salmonella, Klebsiella pneumoniae, Aspergillus sps, Candida and viruses like HIV manipulate host signaling pathways (e.g., NF-κB, MAPK, JAK-STAT) to favor their survival and replication. Topics also include pathogen-induced modulation of programmed cell death pathways (e.g., apoptosis, pyroptosis), and the host’s use of pattern recognition receptors (e.g., TLRs, NLRs) to detect and respond to infection. Host-directed therapies and other emerging therapies for control of pathogens.

Unit 2: Human Microbiome and Symbiotic Interactions

 Composition, diversity, and functional roles of the human microbiome across various body sites such as the gut, skin, oral cavity, and urogenital tract.  Dynamic relationships between the host and commensal microbes, including mechanisms of colonization resistance, nutrient exchange, and modulation of the immune system.  Influence of microbiota on host metabolism, development, and disease susceptibility, including the effects of antibiotics, diet, and probiotics on microbial communities.

Unit 3: Microbial Dysbiosis and Disease: From Inflammation to Therapeutics

Microbiome dysbiosis, and its association with various diseases such as inflammatory bowel disease (IBD), obesity, diabetes, neurodegenerative disorders, and cancer. Methods for analyzing microbial communities (e.g., 16S rRNA sequencing, metagenomics) and  development of microbiome-based diagnostics and therapeutics, including fecal microbiota transplantation (FMT), precision probiotics, and phage therapy. Case studies on how microbial manipulation can restore health or serve as adjuvants in immunotherapy.

Objectives and Outcomes

LEARNING OBJECTIVES

Understand the molecular and cellular strategies used by pathogens to evade and modulate host immune responses.

Explain the role of host signaling pathways and programmed cell death mechanisms in infection and immunity.

Describe the structure, diversity, and function of the human microbiome in health and disease.

Analyze the impact of microbial dysbiosis on chronic and infectious diseases.

Explore modern diagnostic and therapeutic strategies targeting host-pathogen and host-microbiome interactions.

COURSE OUTCOME

After completing the course, students shall be able to  

CO1: Describe mechanisms of immune evasion, intracellular survival, and signaling modulation used by key pathogens such as Mycobacterium tuberculosis, Salmonella, Klebsiella, Candida, and HIV.

CO2: Explain how the human microbiome contributes to immune homeostasis, nutrient metabolism, and resistance to pathogens.

CO3: Evaluate the pathological consequences of microbial dysbiosis and its role in diseases such as IBD, obesity, and cancer.

CO4: Apply knowledge of host-pathogen and microbiome interactions to propose strategies for therapeutic intervention, including host-directed therapies, probiotics, and microbiome transplantation.

CO5: Interpret data from microbiome analysis techniques (e.g., 16S rRNA sequencing, metagenomics) and assess their application in research or clinical settings.

Text Books / References

REFERENCES

  1. The Human Microbiome, Diet, and Health”;Institute of Medicine (US) Food Forum
    Publisher: National Academies Press
  2. The Human Microbiome Handbook; Jason Tetro
    Publisher: Springer
  3. Mims’ Pathogenesis of Infectious Disease” (6th or later dition); Anthony Nash, Richard M. Dalziel, Cedric Mims
    Publisher: Elsevier
  4. “Cellular Microbiology”; Editors: Pascale Cossart, J. Patrice Gorvel, Dominique Ladant
    Publisher: ASM Press

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