Course

1. Micro pipetting.2. Lignin staining: comparison between monocots and dicots.3. Plant and animal cell identification. 4. Mitosis in onion root tip.5. Genomic DNA isolation by CTAB method from different sources like leaf, flowers and fruits of plants.6. Spectrophotometry7. Agarose gel electrophoresis.8. Polyacrylamide gel electrophoresis

LEARNING OBJECTIVES:Hands-on experience of research in Cell Biology. Focuses on using microscopy to investigate various structural features of cells as well as understanding the state of the cells (resting/dividing). Lab also focuses on basic molecular biology techniques including DNA isolation and electrophoresis.COURSE OUTCOMES:After completing the course, students shall be able toCO1: Practical exposure to microscopy wherein the students will learn to differentiate between plant and animal cells and identify the deposition of lignin in plants using various staining techniques.CO2: The various stages of mitosis will be analyzed and visualized using the actively dividing cells present at the root tip of Allium cepa.CO3: Practical exposure to genomic DNA isolation using various plant tissues and standardizing the protocol for each of these tissues.CO4: Understand the method to assess the quality of DNA using Agarose gel electrophoresis and well as spectroscopic methods.CO5: Understand the basis of separation of proteins using polyacrylamide gel electrophoresis.

REFERENCES:1. Cell and Molecular Biology: Concepts and Experiments – Gerald Karp, 3rd Edition, 2006.2. Cell and Molecular Biology: A lab manual -K.V. Chaitanya, 2013.