| Course Name | Cell Culture and Animal Lab |
| Course Code | 25SCR582 |
| Program | M. Sc. Stem Cell Technology and Regenerative Medicine |
| Semester | 1 |
| Credits | 2 |
| Campus | Kochi |
(Lab sessions = 3)
General lay out of a cell culture lab, physical environment needed for the cell culture, growth media and its composition, particularly how the animal use is justified (BSC) and its use in cell culture and how to work in a BSC, organization of cell culture reagents and materials inside the hood to minimize contamination during cell culture and how to control it, culturing and splitting of cell lines, cryopreservation of cells and cell viability assays.
(Lab sessions = 7)
Hands-on cell culture work, media changes, seeding, splitting adherent cells, cell counting, reseeding, and safe disposal
(Lab sessions = 5)
Animal handling techniques, animal feed, gavage, different routes of injection, ethical treatment of animals and Institutional Animal Ethics Committee policies
Pre-requisites: Basic understanding of biology
Total number of lab sessions: 15
Preamble: Cell culture module introduces the students to the basics of cell culture. The course provides students with sufficient knowledge and laboratory skills needed in academia and industry for carrying out basic cell culture techniques properly and safely to perform independently the cell culture technique. The animal handling module introduces students to the regulations, ethics and importance of animal use in research, particularly how the animal use is justified along with basic small animal handling techniques
Course Outcome
CO1: To demonstrate a general level of understanding towards the function, maintenance and working of Bio-safety Cabinets (BSC) and be able to work in BSCs with a good sterilization technique
CO2: To identify culture contamination and methods involved to maintain sterility
CO3: Able to prepare media and maintain adherent cells in culture for atleast a week
CO4: To become aware of standard practices in cell culture and related ethical dilemmas
CO5: Be able to handle small animals and become familiar with ethics involved in animal use for research.
Program Outcomes (PO)
PO1: Bioscience Knowledge
PO2: Problem Analysis
PO3: Design/Development of Solutions
PO4: Conduct Investigations of complex problems
PO5: Modern tools usage
PO6: Bioscientist and Society
PO7: Environment and Sustainability
PO8: Ethics
PO9: Individual & Team work
PO10: Communication
PO11: Project management & Finance
PO12: Lifelong learning
| C | PO1 | PO2 | PO3 | PO4 | PO5 | PO6 | PO7 | PO8 | PO9 | PO10 | PO11 | PO12 |
| CO | ||||||||||||
| CO1 | 3 | 1 | – | – | 3 | – | 3 | – | 1 | 1 | – | 2 |
| CO2 | 2 | 3 | – | – | – | – | 3 | – | 1 | 1 | – | 2 |
| CO3 | 2 | 3 | – | 3 | 3 | – | 3 | – | 3 | 1 | – | 2 |
| CO4 | 2 | 1 | – | – | – | 3 | 3 | 3 | 3 | 2 | – | 2 |
| CO5 | 2 | 1 | – | – | 2 | 3 | 3 | 3 | 3 | 2 | – | 2 |
Program Specific Outcomes. (PSO)
PSO1. Demonstrate comprehensive knowledge of stem cell biology and their clinical and research relevance.
PSO2. Apply core laboratory techniques for stem cell isolation, characterization, and manipulation.
PSO3. Integrate principles of tissue engineering and biomaterials for regenerative applications.
PSO4. Analyze drug delivery, pharmacokinetics, and bioinformatics relevant to stem cell-based therapies.
PSO5. Evaluate ethical, regulatory, and translational aspects of stem cell product development.
PSO6. Bridge basic science with translational approaches in regenerative medicine and gene therapy.
PSO7. Design and interpret experimental strategies for stem cell-based disease
modeling and preclinical studies.
| C | PSO1 | PSO2 | PSO3 | PSO4 | PSO5 | PSO6 | PSO7 |
| C O | |||||||
| CO 1 | – | – | – | – | 1 | – | |
| CO 2 | – | 2 | – | – | 2 | 1 | |
| CO 3 | – | 2 | – | – | 2 | 1 | |
| CO 4 | – | 2 | – | 2 | 3 | 2 | |
| CO 5 | – | – | 1 | – | 3 | 3 | 2 |
Evaluation Pattern: 30+70 = 100
| Internal Assessment – 30% | ||
| Records | Evaluation | 30% |
| 30% | ||
| End Semester Examination- 50% | ||
| Practical | Exam | 60% |
| Assessment | Viva | 10% |
| 70% | ||
| Total | 100% | |
Reference:
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