Course

Unit I

Amino acids, Proteins and PeptidesClassification, stereochemical aspects, physical properties, ionic properties, spectral properties, essential and non-essential amino acids, chemical reactions of amino acids, industrial preparation and chemical synthesis of amino acids. Ionic properties of proteins, protein structure, protein purification, protein structure determination, proteomics and protein function, solid phase peptide synthesis, biologically important peptides.

Unit II

EnzymesIntroduction to enzymes, classification of enzymes, mechanism of enzyme action, immobilized enzymes and enzyme technology, enzyme analogue built polymers, design of molecular clefts, enzymes in synthetic organic chemistry. Enzymes in biological systems

Unit III

Molecular Biology and BioinformaticsStructure of nucleic acids, genes and genome complexity, functions of nucleic acids, isolation and separation of nucleic acids, molecular analysis of nucleic acid sequences, nucleotide sequencing of DNA.

Unit IV

Immunochemical TechniquesProduction of antibodies, purification and fragmentation of immunoglobulins, immunoprecipitation, labelling antibodies, immunoblotting, immunoassays, immunohisto/cytochemistry.

Unit V

Recombinant DNA and Genetic AnalysisConstructing gene libraries, cloning vectors, hybridization and gene probes, application of gene cloning, expression of foreign genes, pharmacogenomics.

REFERENCES1.Hermann Dugas, Bioorganic Chemistry – A Chemical Approach to Enzyme Action, 3rd edition, Springer.2.Keith Wilson and John Walker, Principles and Techniques of Biochemistry and Molecular Biology, 6th edition, Cambridge University Press.