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Course Detail

Course Name Flow Cytometry and Applications
Course Code 25SCR531
Program M. Sc. Stem Cell Technology and Regenerative Medicine
Semester 1
Credits 3
Campus Kochi

Syllabus

Unit 1

Introduction to Flow Cytometry, Overview of flow cytometry and its historical development, Basic components of a flow cytometer: fluidics, optics, and electronics, Principles of fluorescence and fluorochrome selection.

Unit 2

Instrumentation and Operation, Detailed study of flow cytometer components and their functions, Calibration, compensation, and quality control procedures, Hands-on training in instrument startup, operation, and shutdown protocols.

Unit 3

Experimental Design and Multicolor Panel Development, Strategies for designing flow cytometry experiments, Selection of antibodies and fluorochromes for multicolor panels, Controls and standards in flow cytometry assays.

Unit 4

Data Acquisition and Analysis, Techniques for data acquisition and gating strategies, Use of flow cytometry software for data analysis, Interpretation of histograms, dot plots, and statistical outputs.

Unit 5

Cell Sorting Techniques, Principles and mechanics of fluorescence-activated cell sorting (FACS), Sorting strategies: purity vs. yield considerations, Practical sessions on setting up and performing cell sorting experiments.

Unit 6

Applications of Flow Cytometry, Immunophenotyping and analysis of immune cell subsets, Assessment of cell cycle, apoptosis, and proliferation, Applications in stem cell research and cancer immunology.

Introduction

Pre-requisites: Basic understanding of biology

Total number of classes: 45

Credits: 3 (45 classes)

Preamble

Flow Cytometry Cell Sorting and Applications is a graduate-level course offering a comprehensive overview of flow cytometry technologies and their use in research and clinical settings. The course explores principles of fluorescence, antibody labeling, multi-parametric analysis, and cell sorting techniques. Applications discussed include immunophenotyping, cell cycle analysis, apoptosis detection, and stem cell characterization.

Objectives and Outcomes

Course Outcome:

CO1: Understand the principles and components of flow cytometry.

CO2: Operate flow cytometers, including calibration and quality control.

CO3: Design flow cytometry experiments with multicolor panels.

CO4: Acquire and analyze data using gating strategies and flow cytometry software.

CO5: Master FACS techniques for sorting cells based on purity and yield.

CO6: Apply flow cytometry in immunophenotyping, cell cycle analysis, apoptosis, and stem cell research.

Program outcome

PO1: Bioscience Knowledge

PO2: Problem Analysis

PO3: Design/Development of Solutions

PO4: Conduct Investigations of complex problems

PO5: Modern tools usage

PO6: Bioscientist and Society

PO7: Environment and Sustainability

PO8: Ethics

PO9: Individual & Team work

PO10: Communication

PO11: Project management & Finance

PO12: Lifelong learning

0 – No affinity; 1 – low affinity; 2 – Medium affinity; 3 – High affinity

C

PO1

PO2

PO3

PO4

PO5

PO6

PO7

PO8

PO9

PO10

PO11

PO12

CO

CO1

3

2

2

2

3

1

0

1

1

2

1

2

CO2

3

2

2

3

3

1

0

1

1

2

2

2

CO3

3

3

3

3

3

1

0

1

1

3

1

2

CO4

3

3

2

3

3

1

0

1

2

3

1

3

CO5

3

3

3

3

3

2

0

1

2

2

1

3

CO6

3

3

3

3

3

2

0

1

3

3

1

3

Program Specific Outcomes. (PSO)

PSO1. Demonstrate comprehensive knowledge of stem cell biology and their clinical and research relevance.

PSO2. Apply core laboratory techniques for stem cell isolation, characterization, and manipulation.

PSO3. Integrate principles of tissue engineering and biomaterials for regenerative applications.

PSO4. Analyze drug delivery, pharmacokinetics, and bioinformatics relevant to stem cell-based therapies.

PSO5. Evaluate ethical, regulatory, and translational aspects of stem cell product development.

PSO6. Bridge basic science with translational approaches in regenerative medicine and gene therapy.

PSO7. Design and interpret experimental strategies for stem cell-based disease

modeling and preclinical studies.

C

PSO1

PSO2

PSO3

PSO4

PSO5

PSO6

PSO7

C O

CO 1

–

1

–

–

–

1

–

CO 2

–

1

–

–

–

1

–

CO 3

–

2

–

–

–

2

–

CO 4

–

2

–

–

–

2

–

CO 5

–

3

–

–

–

2

1

CO 6

–

3

–

–

–

2

1

Textbook/ Reference

Textbook:

  • Ormerod MG. Flow Cytometry: A Practical Approach. 3rd ed. Oxford: Oxford University Press; 2000.

Reference Book:

  • Robinson JP, Darzynkiewicz Z, Dressler LG, et al., editors. Current Protocols in Cytometry. New York (NY): John Wiley & Sons; 2001.

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