Course

1. Plasmid DNA isolation.2. Agarose gel electrophoresis.3. Competent cell preparation.4. Transformation methods.5. Genomic DNA isolation.6. Restriction digestion of Plasmid DNA.7. Polymerase chain reaction (PCR).8. Poly acrylamide gel electrophoresis and protein analysis.

LEARNING OBJECTIVES:

The course attempts to introduce the basic concepts of recombinant DNA technology namely gene manipulations used for cloning, plasmid and genomic DNA isolation, restriction digestion and analysis by gel electrophoresis and documentation, PCR, transformation techniques, and protein analysis.

COURSE OUTCOMES:

After completing the course, students shall be able to

CO1.Describe basic principles and methodology of Recombinant DNA technology lab like solution preparation, pH measurements, autoclaving, isolation and analysis of genomic DNA and plasmid DNA, PCR, Restriction enzyme digestion, transformation and protein analysis (Knowledge).

CO2.Explains & interprets the results obtained after performing every lab experiment (Understand).

CO3. Applies the knowledge to solve qualitative and quantitative problems like making a PCR mix or designing a double digestion reaction by restriction enzymes, making stock solutions, buffers and using positive and negative controls for different experiments (Apply).

REFERENCES:1. Sambrook, J., Russell, D. W., & Russell, D. W. (2001). Molecular cloning: a laboratory manual (3-volume set).2. Amrita University Virtual Lab (http://vlab.amrita.edu/)