Course

LIST OF EXPERIMENTS:

1. Qualitative analysis of carbohydrates (Sample 1: Glucose)
2. Qualitative analysis of carbohydrates (Sample 2: Fructose)
3. Qualitative analysis of carbohydrates (Sample 3: Lactose)
4. Qualitative analysis of carbohydrates (Sample 4: Maltose)
5. Qualitative analysis of amino acids (Sample 1: Histidine)
6. Qualitative analysis of amino acids (Sample 2: Tryptophan)
7. Qualitative analysis of amino acids (Sample 3: Arginine)
8. Qualitative analysis of amino acids (Sample 4: Tyrosine)
9. Qualitative analysis of abnormal constituents in urine (Albumin, bile salts, ketone bodies, glucose)
10. Estimation of liver function test using the semi-auto analyser
11. Estimation of kidney function test using the semi-auto analyser
12. Estimation of blood urea nitrogen using the semi-auto analyser
13. Estimation of various lipid profiles using the semi-auto analyser
14. Estimation of total protein using the semi-auto analyser

SEMINAR/ASSIGNMENTS:

1. Analysis of Normal Urine Parameters: Students can analyze a dataset of urine analysis results from hospital patients with normal kidney They can calculate and interpret parameters such as pH, specific gravity, protein, glucose, ketones, bilirubin, and microscopic examination findings.

2. Interpretation of Lipid Profile Results: Students can examine lipid profile data from a group of patients and interpret the levels of total cholesterol, HDL cholesterol, LDL cholesterol, and triglycerides.
3. Students can analyze kidney function test results, including serum creatinine, blood urea nitrogen (BUN), and estimated glomerular filtration rate (eGFR), from a dataset of patients. They can discuss the interpretation of these results and their relevance in assessing kidney function.
4. Students can analyze liver function test data, including serum levels of enzymes such as alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), and bilirubin.

TEXTBOOKS:

1. Rodwell VW, Bender D A, Botham K M, Kennelly P J, Weil Harper’s Illustrated Biochemistry. 31^st edition, New York: McGraw Hill, 2022.
2. Vasudevan DM , Sreekumari S, Vaidyanathan K. Textbook of Biochemistry For Medical Students.9^th edition, New Delhi: Jaypee Brothers Medical Publishers, 2019.
3. Satyanarayana U, Chakrapani U. 6^th edition, Elsevier, 2021
4. Sukrutha K, Soumya BA. Text Book of Biochemistry. 1^st edition, Bengaluru: EMMESS Medical Publishers, 2021.

REFERENCE BOOKS:

1. Nelson DL, Cox Lehninger Principles of Biochemistry. 8^th edition, New York: W.
2. Freeman and Company, 2021.
3. Plummer An introduction to practical Biochemistry.3rd edition, India: McGraw Hill, 2017.
4. Gowenlock Varleys Practical Clinical Biochemistry. 6^th edition, India: CBS Publishers, 2006.
5. Pattabhiraman TN. Laboratory Manual & Practical Biochemistry, 4th edition, All India Publishers & Distributors, 2015.

*Latest edition of the text books & reference books can be referred.