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Course Detail

Course Name Molecular Biology Lab
Course Code 25CLG581
Program M. Sc. Clinical Genomics
Semester 1
Credits 2
Campus Kochi

Syllabus

Syllabus

Isolation of chromosomal DNA from Escherichia coli; Isolation of chromosomal DNA from human blood; Isolation of plasmid DNA from Escherichia coli; Nucleic acid quantification; Polymerase chain reaction (PCR); Agarose gel electrophoresis; Restriction digestion, Restriction fragment length polymorphism (RFLP), RNA isolation from human blood; cDNA synthesis, Reverse Transcriptase PCR, Real time PCR; DNA sequencing.

Introduction

Pre-requisites: Basic understanding of DNA chemistry and DNA biology

Total number of classes: 30

Objectives and Outcomes

Course Outcome

CO1 Explain the principles of the DNA & RNA isolation methods, PCR, agarose gel electrophoresis, sequencing methods.

CO2 Can isolate DNA, RNA, plasmids.

CO3 Can perform PCR, cDNA synthesis, RT-PCR, Real-time PCR, and sequencing.

CO4 Can follow general safety routines for laboratory work in molecular biology.

CO5 Can plan experimental work based on a protocol and critically evaluate and discuss experimental results.

*Program Outcomes (PO)

PO1: Bioscience Knowledge

PO2: Problem Analysis

PO3: Design/Development of Solutions

PO4: Conduct Investigations of complex problems

PO5: Modern tools usage

PO6: Bioscientist and Society

PO7: Environment and Sustainability

PO8: Ethics

PO9: Individual & Team work

PO10: Communication

PO11: Project management & Finance

PO12: Lifelong learning

CO–PO Mapping Table:

C O PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11 PO12
CO 1 3 2 2 1 3 1 1 3 1
CO 2 3 2 3 3 3 1 1 3 1
CO 3 3 2 3 3 3 1 1 3 1
CO 4 2 3 3 2 2 1 1
CO 5 3 3 1 2 3 2 1

Program Specific Outcomes (PSO):

PSO1. Apply fundamental molecular biology principles to interpret clinical genomic data.

PSO2. Use molecular techniques (e.g., PCR, RT-PCR, sequencing) to detect genetic mutations and biomarkers.

PSO3. Analyze genotype-phenotype correlations in inherited and acquired disorders.

PSO4. Identify pathogenic variants from NGS data and interpret their clinical relevance.

PSO5. Correlate molecular pathways with disease mechanisms and therapeutic targets.

PSO6. Develop and validate diagnostic assays based on molecular biology principles.

PSO7. Utilize molecular biology to support pharmacogenomic profiling and therapy optimization.

PSO8. Integrate multi-omic data (genomic, transcriptomic, epigenomic) for personalized health solutions.

PSO9. Apply molecular knowledge to cancer genomics, infectious diseases, and rare genetic disorders.

PSO10. Translate molecular discoveries into clinical interventions through evidence-based practice.

CO-PSO Mapping Table:

COs PSO1 PSO2 PSO3 PSO4 PSO5 PSO6 PSO7 PSO8 PSO9 PSO10
CO1 3 3 2 2 2 3 2 2 1 2
CO2 2 3 1 3 1 1
CO3 3 3 2 3 2 3 2 2 2 3
CO4 1 2 2 1 1
CO5 3 3 2 3 3 3 2 2 2 3

Evaluation Pattern

Evaluation Pattern: 30+70 = 100

Internal Assessment – 30%
Records Evaluation 30%
30%
End Semester Examination – 50%
Practical Exam 60%
Assessment Viva 10%
70%

Textbooks / References

Textbooks

  1. Joseph Sambrook, David William Russell, Molecular Cloning: A Laboratory Manual, Volume 1-2-3 CSHL Press, 2001.
  2. Terence A. Brown, Essential Molecular Biology: A Practical Approach, Volume 1 Oxford University Press, 2000.
  3. Udo Reischl; Molecular Diagnostics of infectious diseases; Humana Press.
  4. Adrian J Harwood, Methods in Molecular Biology; Protocols for gene analysis.
  5. Frederick M Ausubel, Roger Brent, Robert D Moore, J G Seidman, John A smith, Kevin Struhl; Current protocols in Molecular Biology, John Wiley and Sons, Inc (Volume 1-4).

References

Gene Therapy Technologies, Applications and Regulations: From Laboratory to Clini. Anthony Meager 1999 John Wiley & Sons, Ltd.

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