Qualification: 
MSc
ajithm@am.amrita.edu

Ajith M. currently serves as Assistant Professor at the School of Biotechnology at Amritapuri campus. He received his masters in Applied Microbiology from PSG College of Arts & Science, Coimbatore.

TEACHING

Publications

Publication Type: Journal Article

Year of Publication Publication Type Title

2017

Journal Article

Chandni P, Amrita Salim, Archana P. V., Pradeesh Babu, Dr. Bipin G. Nair, Madhavan, A., and Dr. Sanjay Pal, “Characterization of the bacteriophages binding to human matrix molecules.”, International Journal of Biological Macromolecules, 2017.[Abstract]


Recent literature has suggested a novel symbiotic relationship between bacteriophage and metazoan host that provides antimicrobial defense protecting mucosal surface by binding to host matrix mucin glycoproteins. Here, we isolated and studied different bacteriophages that specifically interact with human extracellular matrix molecules such as fibronectin, gelatin, heparin and demonstrated their potency for protection to host against microbial infections. We showed that subpopulations of bacteriophages that work against clinical isolates of Escherichia coli can bind to pure gelatin, fibronectin and heparin and reduced bacterial load in human colon cell line HT29. The bacteriophages were characterized with respect to their genome sizes, melting curve patterns and host tropism (cross-reactivity with different hosts). Since, the bacteriophages are non-toxic to the host and can effectively reduce bacterial load in HT29 cell line their therapeutic potency against bacterial infection could be explored.

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2016

Journal Article

A. Madhavan, Pandurangan Nanjan, K. Shetty, S., and Shanmugham, S., “A rapid and selective method for the quantification of naringenin in order to monitor naringinase activity”, International Journal of ChemTech Research, vol. 9, pp. 333-338, 2016.[Abstract]


Sodium acetate (NaOAc) can selectively ionize 7-OH of naringenin generating a bathochromic shift of 41nm in its UV absorbance. This principle is used in the described spectrophotometric method that can detect naringenin amidst naringin and prunin present in the incubation mixture of naringinase. The method could be adopted for real sample analysis as it remains unaffected by the presence of phenolics such as ascorbic acid, gallic acid, citric acid and cinnamic acid. © 2016, Sphinx Knowledge House.

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Publication Type: Conference Paper

Year of Publication Publication Type Title

2017

Conference Paper

V. Amrutha., Prasad Megha, Lekshmija, A., Anjana, R., Aleena, S., Dr. Bipin G. Nair, Madhavan, A., and Dr. Sanjay Pal, “Effect of compost derived lytic agents against enteric bacteria in sewage”, in InnovativeStrategies for Sustainable Water Management (ISSWM-2017), Punjab, India. , 2017.

2017

Conference Paper

Pradeesh Babu, Poornendu, S. J., Amrita Salim, Madhavan, A., Dr. Bipin G. Nair, and Dr. Sanjay Pal, “Resazurin based redox dye as an indicator for monitoring wastewater biological activity”, in Innovative Strategies for Sustainable Water Management (ISSWM-2017), Punjab, India, 2017.

2017

Conference Paper

M. Sreejith, Reghu, A. P., Anandakrishnan, K., Gopika, P. J., .Kuriakose, G., Reshma, M. J., Vishnu, K., Madhavan, A., Dr. Bipin G. Nair, and Dr. Sanjay Pal, “Screening potential antimicrobial compounds by resazurin dye based viability assay”, in Innovative Strategies for Sustainable Water Management (ISSWM-2017), Punjab, India, 2017.

2017

Conference Paper

D. Tharuvana, A. Sundaresh, Sreelakshmi, V. J., Das, A., Dr. Bipin G. Nair, Dr. Sanjay Pal, and Madhavan, A., “Sand and charcoal as matrices for immobilization of phages for wastewater treatment”, in Innovative Strategies for Sustainable Water Management (ISSWM-2017) , Punjab, India. , 2017.

2017

Conference Paper

A. P. Veedu, Reshma, R. N., Dr. Bipin G. Nair, Dr. Sanjay Pal, and Madhavan, A., “Activity of probiotic strains against enteric pathogens”, in Innovative Strategies for Sustainable Water Management (ISSWM-2017) , Punjab,India. , 2017.

2017

Conference Paper

A. Madhavan, Prasad Megha, Girish, S., K. Shetty, S., Dr. Bipin G. Nair, and Dr. Sanjay Pal, “Caulobacter crescentus as a novel exoelectricigen in a dual chambered Microbial Fuel Cell (MFC)”, in Technical Program Committee of the “2017 IEEE International( Biennial) Conference on Technological Advancements in Power and Energy”, TAP Energy-2017. , 2017.

2017

Conference Paper

A. Madhavan, K. Shetty, S., Anjana, G., Das, A., Athira, A. S., Hari, H., ,, Babu, S., Dr. Bipin G. Nair, and Dr. Sanjay Pal, “Pneumatophore inspired biomimetic approach to design an energy neutral aerator for application in composting”, in Technical Program Committee of the “2017 IEEE International ( Biennial) Conference on Technological Advancements in Power and Energy”, TAP Energy-2017. , 2017.

2017

Conference Paper

P. Nagarajan, Sruthy, K. S., Lal, V. P., Devan, V. P., Krishna, A., Lakshman, A., Vineetha, K. M., Dr. Bipin G. Nair, Dr. Sanjay Pal, and Madhavan, A., “Biological treatment of domestic wastewater by selected aquatic plants”, in IEEE International Conference on Technological Advancements in Power & Energy (TAP Energy 2017). , 2017.[Abstract]


Around 30 percent of the operating costs of conventional centralized wastewater treatment is required for energy to operate the machines for pump and aeration. Comparatively, constructed wetlands involving photosynthetic plants and other organisms have a lower energy requirement. The aim of the present work is to select aquatic plants which have high efficiency of removing infection in wastewater. Four plants were chosen from locally available plants growing in wastewater. They were put in raw wastewater from toilets for 24 h and were tested for their survival and growth measured by chlorophyll content. Brahmi (Bacopa monnieri) and Duckweed were found to be best compared to Azolla and Hydrilla. The efficiency of removal of enteric infections was done by culturing the treated wastewater on Eosin Methylene Blue (EMB) agar media, a selective and differential medium for enteric gram negative bacteria. The best performing plants were Brahmi and Hydrilla which reduced the load by 67-70%. But Hydrilla did not grow as well compared to Brahmi as evident from the chlorophyll content. Hence Brahmi and Duckweed can be considered as most effective for removal of infection in wastewater among the four tested plants and potentially may save energy and synthetic chemicals otherwise required for conventional treatment systems. Brahmi, famous for its high value medicinal compounds against neuronal problems, may be used for extraction of the compounds. The remaining biomass can be used as feedstock in anaerobic digester for methane/ethanol generation to be used as biofuel.

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2016

Conference Paper

A. Madhavan, Nandakumar, V., K. Shetty, S., Dr. Bipin G. Nair, and Dr. Sanjay Pal, “Optimization of microbial fuel cell (MFC) operated with waste water as substrate”, in Electrical, Electronics, and Optimization Techniques (ICEEOT), International Conference on, Chennai, India, 2016.[Abstract]


In the present study bioelectricity generation from waste water was evaluated in a double chambered microbial fuel cell (MFC). Waste water and potassium permanganate solution (0.3%) was loaded in the anodic and the cathodic chamber respectively. The performance of MFC was studied with two different electrodes (aluminum and carbon as anodes and copper as cathode). Current and voltage measurements were carried out using Keithley source meter (2420) and multimeter respectively. The Voltammetric analysis was done to study the anodic oxidation rate. The resistance of the system was found to be low (0.466 Ω for the aluminum anode and 0.673 Ω carbon anode) which were deduced from the power density curves. The system showed a COD removal of 77% over a period of a week ofk operation.

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2013

Conference Paper

A. Madhavan, S, S., Bos, C., and Pandurangan, N., “Screening, Production, Purification and Application of Naringenase Elaborated by Aspergillus Species of Section Nilgri”, in International Conference on Biotechnology for Innovative Applications, Amrita Vishwa Vidyapeetham, Kollam, Kerala, India, 2013.

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