Qualification: 
MSc
ajithm@am.amrita.edu

Ajith M. currently serves as Assistant Professor at the School of Biotechnology at Amritapuri campus. He received his masters in Applied Microbiology from PSG College of Arts & Science, Coimbatore.

TEACHING

Publications

Publication Type: Journal Article

Year of Publication Title

2019

Amrita Salim, Babu, P., Mohan, K., Moorthy, M., Raj, D., Thirumeni, S. Kallampill, Suresh, S., Ajith Madhavan, Dr. Bipin G. Nair, Chattopadhyay, S., and Dr. Sanjay Pal, “Draft Genome Sequence of an Escherichia coli Sequence Type 155 Strain Isolated from Sewage in Kerala, India”, Microbiology Resource Announcements, vol. 8, 2019.[Abstract]


We report the draft genome sequence of Escherichia coli ASBT-1, a representative of E. coli sequence type 155 (ST155), obtained from India. Considering the known wide variety of pathogenic and antibiotic resistance potentials, this strain should be of great interest for detailed comparative genomic analysis.

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2018

S. Meppoyilam, Chinchu Bose, Reghu, A. P., K, A., P.J., G., Kuriakose, G., M.J., R., K., V., Ajith Madhavan, Dr. Bipin G. Nair, and Dr. Sanjay Pal, “Quick screening of extract from plant waste for control of specific pathogens in wastewater by resazurin assay”, Pollution Research, vol. 37, pp. 160-165, 2018.[Abstract]


Biological control of infection in wastewater and compost is posed to be ecologically and economically very attractive but needs a detailed understanding of the complex interaction of the abiotic and biotic factors controlling infection. We intend to screen different cheap natural compounds from plant biomass waste which can effectively control infection in wastewater/ compost. Resazurin (alamarBlue®) based viability method is a sensitive and cost effective assay which can be adopted to screen antimicrobial compounds with high throughput compared to culture based methods. However, the assay needs to be standardized for each type of bacterial species as their metabolic activity varies widely. Antimicrobial assay generally requires substantial (12-24 h) time for both culture and fluorescence based assay. In the present study, resazurin assay was standardized for Salmonella typhi and Staphylococcus aureus where time required was reduced to 4 h. This method was utilized for the screening of plant extracts (Cocos nucifera, Areca catechu and Allium cepa) from plant waste material against target microorganisms. Among the extracts tested, MEK (Methyl ethyl ketene) extract of onion peel (Allium cepa) showed maximum (2-log) reduction in relative fluorescence unit (RFU) for both Salmonella typhi and Staphylococcus aureus. These results were compared with conventional culture based techniques as well. Thus our resazurin based assay method shows a good promise for rapid screening of the compounds against the two important human pathogens, S. typhi and S. aureus.

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Publication Type: Patent

Year of Publication Title

2018

S. Pal, Dr. Bipin G. Nair, Ajith Madhavan, Pradeesh Babu, Amrita Salim, P., C., Stanely, J., and Babu, S., “Methods and devices for detecting antimicrobial resistant bacteria using bacteriophages”, U.S. Patent 2017410206692018.

Publication Type: Conference Paper

Year of Publication Title

2018

D. Tharuvana, A. Sundaresh, V. J. Sreelakshmi, A. Das, Dr. Bipin G. Nair, Dr. Sanjay Pal, and Ajith Madhavan, “Sand and charcoal as matrices for immobilization of phages for wastewater treatment”, in Innovative Strategies for Sustainable Water Management (ISSWM-2017) , Punjab, India. , 2018.

2018

Pradeesh Babu, S. J. Poornendu, Amrita Salim, Ajith Madhavan, Dr. Bipin G. Nair, and Dr. Sanjay Pal, “Resazurin based redox dye as an indicator for monitoring wastewater biological activity”, in Innovative Strategies for Sustainable Water Management (ISSWM-2017), Punjab, India, 2018.

2018

V. Amrutha., Prasad Megha, A. Lekshmija, R Anjana, S. Aleena, Dr. Bipin G. Nair, Ajith Madhavan, and Dr. Sanjay Pal, “Effect of compost derived lytic agents against enteric bacteria in sewage”, in InnovativeStrategies for Sustainable Water Management (ISSWM-2017), Punjab, India. , 2018.

2017

P. Nagarajan, K. S. Sruthy, V. P. Lal, V. P. Devan, A. Krishna, A. Lakshman, K M Vineetha, Dr. Bipin G. Nair, Dr. Sanjay Pal, and Ajith Madhavan, “Biological treatment of domestic wastewater by selected aquatic plants”, in IEEE International Conference on Technological Advancements in Power & Energy (TAP Energy 2017). , 2017.[Abstract]


Around 30 percent of the operating costs of conventional centralized wastewater treatment is required for energy to operate the machines for pump and aeration. Comparatively, constructed wetlands involving photosynthetic plants and other organisms have a lower energy requirement. The aim of the present work is to select aquatic plants which have high efficiency of removing infection in wastewater. Four plants were chosen from locally available plants growing in wastewater. They were put in raw wastewater from toilets for 24 h and were tested for their survival and growth measured by chlorophyll content. Brahmi (Bacopa monnieri) and Duckweed were found to be best compared to Azolla and Hydrilla. The efficiency of removal of enteric infections was done by culturing the treated wastewater on Eosin Methylene Blue (EMB) agar media, a selective and differential medium for enteric gram negative bacteria. The best performing plants were Brahmi and Hydrilla which reduced the load by 67-70%. But Hydrilla did not grow as well compared to Brahmi as evident from the chlorophyll content. Hence Brahmi and Duckweed can be considered as most effective for removal of infection in wastewater among the four tested plants and potentially may save energy and synthetic chemicals otherwise required for conventional treatment systems. Brahmi, famous for its high value medicinal compounds against neuronal problems, may be used for extraction of the compounds. The remaining biomass can be used as feedstock in anaerobic digester for methane/ethanol generation to be used as biofuel.

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2017

Ajith Madhavan, Sindhu Shetty K., G. Anjana, A. Das, A.S. Athira, H. Hari, K. S. Lekshmi, S. Babu, Dr. Bipin G. Nair, and Dr. Sanjay Pal, “Pneumatophore inspired biomimetic approach to design an energy neutral aerator for application in composting”, in Technical Program Committee of the “2017 IEEE International ( Biennial) Conference on Technological Advancements in Power and Energy”, TAP Energy-2017. , 2017.

2017

Ajith Madhavan, Prasad Megha, S. Girish, Sindhu Shetty K., Dr. Bipin G. Nair, and Dr. Sanjay Pal, “Caulobacter crescentus as a novel exoelectricigen in a dual chambered Microbial Fuel Cell (MFC)”, in Technical Program Committee of the “2017 IEEE International( Biennial) Conference on Technological Advancements in Power and Energy”, TAP Energy-2017. , 2017.

2016

Ajith Madhavan, Vishnu Nandakumar, Sindhu Shetty K., Dr. Bipin G. Nair, and Dr. Sanjay Pal, “Optimization of microbial fuel cell (MFC) operated with waste water as substrate”, in Electrical, Electronics, and Optimization Techniques (ICEEOT), International Conference on, Chennai, India, 2016.[Abstract]


In the present study bioelectricity generation from waste water was evaluated in a double chambered microbial fuel cell (MFC). Waste water and potassium permanganate solution (0.3%) was loaded in the anodic and the cathodic chamber respectively. The performance of MFC was studied with two different electrodes (aluminum and carbon as anodes and copper as cathode). Current and voltage measurements were carried out using Keithley source meter (2420) and multimeter respectively. The Voltammetric analysis was done to study the anodic oxidation rate. The resistance of the system was found to be low (0.466 Ω for the aluminum anode and 0.673 Ω carbon anode) which were deduced from the power density curves. The system showed a COD removal of 77% over a period of a week ofk operation.

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2013

Ajith Madhavan, S, S., Bos, C., and Pandurangan, N., “Screening, Production, Purification and Application of Naringenase Elaborated by Aspergillus Species of Section Nilgri”, in International Conference on Biotechnology for Innovative Applications, Amrita Vishwa Vidyapeetham, Kollam, Kerala, India, 2013.

2013

Ajith Madhavan, S, S., Chinchu Bose, and Pandurangan Nanjan, “Screening, Production, Purification and Application of Naringenase Elaborated by Aspergillus Species of Section Nigri”, in International Conference on Biotechnology for Innovative Applications, Amrita Vishwa Vidyapeetham, Kollam, Kerala, 2013.