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Evaluation of Innate Lymphoid Cells and Dendritic Cells Following Viral Vector Vaccination

Publication Type : Book Chapter

Publisher : Springer

Source : Vaccine Technologies for Veterinary Viral Diseases: Methods and Protocols

Url : https://link.springer.com/protocol/10.1007/978-1-0716-2168-4_8

Campus : Faridabad

Center : Amrita Research Center Delhi

Year : 2022

Abstract : Recently, we have shown that fate of a vaccine is determined by the cytokine milieu in the innate immune compartment at the early stage of vaccination. Specifically, 24 h post-delivery, level of innate lymphoid cell type 2 ILC2-derived IL-13/IL-13Rα2 are the master regulators of DC and also different ILC subsets responsible for modulating the downstream immune outcomes. Here, we provide step-by-step details how to assess different ILC and DC subsets in lung and muscle following intranasal and intramuscular viral vector vaccination, respectively, using multi-color flow cytometry and confocal microscopy.

Cite this Research Publication : Li Z, Roy S, Ranasinghe C, Evaluation of Innate Lymphoid Cells and Dendritic Cells Following Viral Vector Vaccination, Vaccine Technologies for Veterinary Viral Diseases: Methods and Protocols, Springer US New York, NY; 2022. p. 137-53.

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