Publication

Abstract : This study was an attempt to fabricate an antibacterial wound dressing, which was a bilayered polycaprolactone / polyvinyl alcohol-chitosan (PCL/PVA-CS) nanofibrous membrane. Entrapping ethanolic leaf extract of Tridax procumbens L. (PCL/PVA-CS/Tp). The membrane was prepared using the electrospinning technique to obtain beadless uniform nanofibers. The extract was then infused into the membrane by spraying and exposing to high temperature. in vitro antibacterial activity and cell viability of the membranes were performed. An optimized concentration of 800 μg. mL−1 of Tp extract in PCL/PVA-CS/Tp evinced better antibacterial effect on E. coli than S. aureus and also showed rapid wound closure with a positive impact on the viability of L929 cell line. The tissue regeneration efficacy of PCL/PVA-CS/Tp was validated by the experiments on mice models with subcutaneous wounds created using biopsy punch and laser radiation causing burns. Furthermore, in vivo assessments illustrated that the biopsy punch wounds healed more rapidly than laser burn though healing was significant in both. The healing processes such as anti-inflammation and re-epithelialization were observed through histological study. The upregulation of proteins namely VEGF and CD31 along with a decrease in protein levels of Wnt and TGF-β indicated significant wound healing. In conclusion, the bilayered membrane infused with plant extract could be considered a potential material for wound dressing.