Unit 1
EnzymesEnzyme nomenclature, enzyme commission numbers, enzymes in bioanalytical chemistry, enzyme kinetics-enzyme activators, enzyme inhibitors.
Unit 2
Quantification of Enzymes and Their SubstratesInstrumental methods, optical detection – absorbance, fluorescence, luminescence, nephelometry electrochemical detection – amperometry, potentiometry, conductometry, other detection methods – radiochemical, manometry, calorimetry.
Unit 3
Immobilized EnzymesImmobilization methods – nanopolymerizing covalent immobilization, Crosslinking with bifunctional reagents. Properties of immobilized enzymes, immobilized enzyme reactions, theoretical treatment of packed bed enzyme reactors.
Unit 4
AntibodiesStructural and functional properties of antibodies, Polyclonal and monoclonal antibodies Antibody-antigen interactions, analytical application of secondary antibody-antigen.
Unit 5
BiosensorsResponse of enzyme-based biosensors, examples of biosensor configuration, ferrocene-mediated amperometric glucose sensor, potentiometric biosensor for phenyl acetate, potentiometric immunosensor for digoxin, optical biosensor for glucose based on fluorescence energy transfer, piezoelectric sensor for nucleic acid detection, enzyme thermistors.