Unit 1
Introduction to biosensor classification based on the signal transduction and biorecognition element. Enzymatic and non-enzymatic sensors, DNA and protein-based sensors – immunosensors.
Unit 2
Biosensing using nanomaterials: concepts of surface to volume ratio, quantum confinement, surface plasmon resonance, fluorescence, chemiluminescence and electroluminescence and FRET in biosensing. Application of metal, semiconducting quantum dots, carbon nanotubes, graphene and carbon dots in biosensing.
Unit 3
Electrochemical principle in biosensing: principles of potentiometry, voltammetry, amperometry and impedimentary in biosensing. Principle, fabrication and working of optical, electrochemical biosensors. Construction and working of potentiometric, amperometric and impedemetric sensors. Development and applications of piezoelectric sensors.
Unit 4
Optical and electrochemical sensors for glucose, vitamins, cholesterol, dopamine, nitric oxide, nitrates, and pesticides. Biocompatibility of sensors.
Unit 5
Biochips and wearable devices: lab-on-a-chip – fabrication of microfluidics- lithography, wearable sensors, epidermal electronic system, lab-on-skin-devices.