Prescribed Syllabus
Lab Precautions, Sterilization, Micrometry, Capsular Staining , Motility determination-Soft agar deeps and Hanging drop method, Biochemical tests: IMViC test,Urease test, Catalase test, Oxidase test, carbohydrate fermentation separately and Triple sugar iron test, Anaerobic culture techniques-Wrights tube method, Ribotyping (demo)
| Sl. No. | TOPICS TO BE COVERED | NO. OF SESSIONS ALLOTED |
| 1. | Lab Precautions and Sterilization | 3h |
| 2. | Pure Culture Techniques-Spread Plate and Pour Plate | 3h |
| 3. | Micrometry | 3h |
| 4. | Motility determination-Soft agar deeps and Hanging drop method | 3h |
| 5. | Staining techniques- Gram staining | 3h |
| 6. | Capsular Staining | 3h |
| 7. | Biochemical tests: Indole and methyl red test | 3h |
| 8. | Voges-Proskaur and Citrate test | 3h |
| 9. | Catalase and Oxidase test | 3h |
| 10. | Urease test | 3h |
| 11. | Triple sugar Iron Agar test + separate sugar fermentation tests | 3h |
| 12. | Anaerobic culture techniques-Wrights tube method | 3h |
| 13. | Ribotyping | 3h |